TAF5/YBR198C Summary Help

Standard Name TAF5 1
Systematic Name YBR198C
Alias TAF90
Feature Type ORF, Verified
Description Subunit (90 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification (1, 2, 3 and see Summary Paragraph)
Also known as: TafII90 4
Name Description TATA binding protein-Associated Factor
Chromosomal Location
ChrII:618523 to 616127 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All TAF5 GO evidence and references
  View Computational GO annotations for TAF5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
611 total interaction(s) for 305 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 335
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 80
  • Biochemical Activity: 2
  • Co-localization: 7
  • Co-purification: 20
  • Far Western: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Rescue: 1
  • Negative Genetic: 120
  • Positive Genetic: 21
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 5

Expression Summary
Length (a.a.) 798
Molecular Weight (Da) 88,967
Isoelectric Point (pI) 7.39
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:618523 to 616127 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2397 618523..616127 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000402


TFIID is a transcription factor complex that is required for RNAPII-mediated transcription of protein-coding genes and some small nuclear RNAs (reviewed in 5). The complex is composed of Spt15p (TATA binding protein; TBP) and 14 TBP-associated factors (TAFs): Taf1p, Taf2p, Taf3p, Taf4p, Taf5p, Taf6p, Taf7p, Taf8p, Taf9p, Taf10p, Taf11p, Taf12p, Taf13p, Taf14p (6, 7). The TFIID complex is required for basal transcription, but some individual subunits regulate the activated transcription of a subset of genes (8, 9, 10, 11, 12).

Recognition of promoter DNA by the TFIID complex is required for the formation of the preinitiation complex (PIC) during transcription initiation (13, 14). The interaction between the TFIID complex and the promoter is stabilized by TFIIA (14, 15). The recruitment of TFIID to promoters is dependent on an upstream activating sequence in the promoter region (16).

A subset of the TAFs (Taf5p, Taf6p, Taf9p, Taf10p, and Taf12p) are subunits of both TFIID and the the Spt-Ada-Gcn5-acetyltransferase (SAGA) transcriptional regulatory complex, which functions in nucleosomal histone acetylation and chromatin-associated transcriptional activation or repression (2, 17, 18). The results of genome-wide studies indicate that TFIID functions primarily at the TATA-less promoters of stress-repressed housekeeping genes, representing about 90% of the yeast genome, while SAGA predominates at highly-regulated, stress-responsive TATA box-containing genes, representing about 10% of the genome (19, 20).

Last updated: 2009-10-02 Contact SGD

References cited on this page View Complete Literature Guide for TAF5
1) Tora L  (2002) A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription. Genes Dev 16(6):673-5
2) Grant PA, et al.  (1998) A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation. Cell 94(1):45-53
3) Lee TI and Young RA  (2000) Transcription of eukaryotic protein-coding genes. Annu Rev Genet 34:77-137
4) Reese JC, et al.  (1994) Yeast TAFIIS in a multisubunit complex required for activated transcription. Nature 371(6497):523-7
5) Tansey WP and Herr W  (1997) TAFs: guilt by association? Cell 88(6):729-32
6) Sanders SL, et al.  (2002) Molecular characterization of Saccharomyces cerevisiae TFIID. Mol Cell Biol 22(16):6000-13
7) Auty R, et al.  (2004) Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem 279(48):49973-81
8) Sayre MH, et al.  (1992) Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae. J Biol Chem 267(32):23376-82
9) Macpherson N, et al.  (2000) A yeast taf17 mutant requires the Swi6 transcriptional activator for viability and shows defects in cell cycle-regulated transcription. Genetics 154(4):1561-76
10) Kobayashi A, et al.  (2003) Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP). Nucleic Acids Res 31(4):1261-74
11) Klebanow ER, et al.  (1996) Isolation and characterization of TAF25, an essential yeast gene that encodes an RNA polymerase II-specific TATA-binding protein-associated factor. J Biol Chem 271(23):13706-15
12) Walker SS, et al.  (1997) Yeast TAF(II)145 required for transcription of G1/S cyclin genes and regulated by the cellular growth state. Cell 90(4):607-14
13) Shen WC, et al.  (2003) Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly. EMBO J 22(13):3395-402
14) Buratowski S, et al.  (1989) Five intermediate complexes in transcription initiation by RNA polymerase II. Cell 56(4):549-61
15) Ranish JA and Hahn S  (1991) The yeast general transcription factor TFIIA is composed of two polypeptide subunits. J Biol Chem 266(29):19320-7
16) Li XY, et al.  (2002) Selective recruitment of TAFs by yeast upstream activating sequences. Implications for eukaryotic promoter structure. Curr Biol 12(14):1240-4
17) Pray-Grant MG, et al.  (2002) The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway. Mol Cell Biol 22(24):8774-86
18) Warfield L, et al.  (2004) Positive and negative functions of the SAGA complex mediated through interaction of Spt8 with TBP and the N-terminal domain of TFIIA. Genes Dev 18(9):1022-34
19) Huisinga KL and Pugh BF  (2004) A genome-wide housekeeping role for TFIID and a highly regulated stress-related role for SAGA in Saccharomyces cerevisiae. Mol Cell 13(4):573-85
20) Basehoar AD, et al.  (2004) Identification and distinct regulation of yeast TATA box-containing genes. Cell 116(5):699-709