SPT7/YBR081C Summary Help

Standard Name SPT7 1
Systematic Name YBR081C
Alias GIT2 2
Feature Type ORF, Verified
Description Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex (3, 4, 5 and see Summary Paragraph)
Name Description SuPpressor of Ty's 1
Chromosomal Location
ChrII:405251 to 401253 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 46 cM
Gene Ontology Annotations All SPT7 GO evidence and references
  View Computational GO annotations for SPT7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 1135 genes
Regulators 72 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
496 total interaction(s) for 214 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 319
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 87
  • Biochemical Activity: 3
  • Co-fractionation: 2
  • Co-localization: 2
  • Co-purification: 16
  • Reconstituted Complex: 6
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 23
  • Synthetic Lethality: 25
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,332
Molecular Weight (Da) 152,616
Isoelectric Point (pI) 4.57
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:405251 to 401253 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 46 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3999 405251..401253 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000285
SUMMARY PARAGRAPH for SPT7

SPT7 encodes a subunit of SAGA, a multi-subunit coactivator complex that regulates transcription of some RNA polymerase II-dependent genes (3). A C-terminally truncated form of Spt7p is also found in an altered SAGA complex, termed SLIK (5) or SALSA (4), which lacks Spt8p and full-length Spt7p. Mutant analysis indicates that the C-terminal region of Spt7p is required for assembly of Spt8p into SAGA. In addition, Spt7p is required for normal amounts of two other SAGA components required for SAGA integrity, Spt20p and Ada1p, suggesting that Spt7p plays a critical role in SAGA complex formation (6, 7).

SPT7 was originally identified in a genetic screen for mutations that suppress Ty or solo Delta element insertions in the HIS4 or LYS2 promoters (1, 8). spt7 null mutant phenotypes include: slow growth, inositol auxotrophy, altered cell morphologies relative to wild-type cells, defects in sporulation, and defects in transcription of Ty elements (9). Many of the null mutant phenotypes are complemented by expression of the carboxy-terminal 459 amino acids of SPT7 (1332 amino acids long). Interestingly, the 414 amino acid human protein STAF65gamma, which is a component of the SAGA-related STAGA complex, is homologous to the carboxy-terminal 543 amino acids of Spt7p (10).

Characterized subunits of the SAGA complex include: Hfi1p, Ada2p, Ngg1p, Spt20p, Gcn5p, Spt3p, Spt7p, Spt8p, Tra1p, Taf5p, Taf6p, Taf9p, Taf10p, and Taf12p, Ubp8p, and Sgf11p (11, 12; reviewed in 4)

Last updated: 2006-07-03 Contact SGD

References cited on this page View Complete Literature Guide for SPT7
1) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2) Patton-Vogt JL and Henry SA  (1998) GIT1, a gene encoding a novel transporter for glycerophosphoinositol in Saccharomyces cerevisiae. Genetics 149(4):1707-15
3) Grant PA, et al.  (1997) Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex. Genes Dev 11(13):1640-50
4) Sterner DE, et al.  (2002) SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. Proc Natl Acad Sci U S A 99(18):11622-7
5) Pray-Grant MG, et al.  (2002) The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway. Mol Cell Biol 22(24):8774-86
6) Sterner DE, et al.  (1999) Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol 19(1):86-98
7) Wu PY and Winston F  (2002) Analysis of Spt7 function in the Saccharomyces cerevisiae SAGA coactivator complex. Mol Cell Biol 22(15):5367-79
8) Winston F, et al.  (1987) Three genes are required for trans-activation of Ty transcription in yeast. Genetics 115(4):649-56
9) Gansheroff LJ, et al.  (1995) The Saccharomyces cerevisiae SPT7 gene encodes a very acidic protein important for transcription in vivo. Genetics 139(2):523-36
10) Martinez E, et al.  (2001) Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. Mol Cell Biol 21(20):6782-95
11) Henry KW, et al.  (2003) Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes Dev 17(21):2648-63
12) Lee KK, et al.  (2005) The deubiquitylation activity of Ubp8 is dependent upon Sgf11 and its association with the SAGA complex. Mol Cell Biol 25(3):1173-82