SED5/YLR026C Summary Help

Standard Name SED5 1
Systematic Name YLR026C
Feature Type ORF, Verified
Description cis-Golgi t-SNARE syntaxin; required for vesicular transport between the ER and the Golgi complex; binds at least 9 SNARE proteins (2, 3, 4, 5, 6)
Name Description Suppressor of Erd2 Deletion 1
Chromosomal Location
ChrXII:196472 to 195450 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SED5 GO evidence and references
  View Computational GO annotations for SED5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
reduction of function
240 total interaction(s) for 100 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 78
  • Co-crystal Structure: 1
  • Co-fractionation: 3
  • Co-purification: 44
  • PCA: 2
  • Reconstituted Complex: 42
  • Two-hybrid: 21

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 10
  • Negative Genetic: 13
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 7
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 340
Molecular Weight (Da) 38,807
Isoelectric Point (pI) 6.99
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:196472 to 195450 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1023 196472..195450 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004016
References cited on this page View Complete Literature Guide for SED5
1) Hardwick KG, et al.  (1992) Genes that allow yeast cells to grow in the absence of the HDEL receptor. EMBO J 11(11):4187-95
2) Hardwick KG and Pelham HR  (1992) SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex. J Cell Biol 119(3):513-21
3) Peng R, et al.  (1999) Specific interaction of the yeast cis-Golgi syntaxin Sed5p and the coat protein complex II component Sec24p of endoplasmic reticulum-derived transport vesicles. Proc Natl Acad Sci U S A 96(7):3751-6
4) Sacher M, et al.  (1997) The synaptobrevin-related domains of Bos1p and Sec22p bind to the syntaxin-like region of Sed5p. J Biol Chem 272(27):17134-8
5) Grabowski R and Gallwitz D  (1997) High-affinity binding of the yeast cis-Golgi t-SNARE, Sed5p, to wild-type and mutant Sly1p, a modulator of transport vesicle docking. FEBS Lett 411(2-3):169-72
6) Tsui MM, et al.  (2001) Selective formation of Sed5p-containing SNARE complexes is mediated by combinatorial binding interactions. Mol Biol Cell 12(3):521-38