SNF12/YNR023W Summary Help

Standard Name SNF12 1
Systematic Name YNR023W
Alias SWP73 2 , 3
Feature Type ORF, Verified
Description 73 kDa subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive; SNF12 has a paralog, RSC6, that arose from the whole genome duplication (1, 2, 4, 5, 6, 7 and see Summary Paragraph)
Name Description Sucrose NonFermenting 8
Chromosomal Location
ChrXIV:670418 to 672118 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SNF12 GO evidence and references
  View Computational GO annotations for SNF12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
conditional
null
Large-scale survey
null
Resources
70 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 33
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 16
  • Co-crystal Structure: 1
  • Co-purification: 2
  • Reconstituted Complex: 7

Genetic Interactions
  • Dosage Lethality: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 566
Molecular Weight (Da) 63,870
Isoelectric Point (pI) 5.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:670418 to 672118 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1701 670418..672118 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005306
SUMMARY PARAGRAPH for SNF12

Snf12p is a component of SWI/SNF (3, 9), and is required for maintaining the full structural integrity of the complex (10). Snf12p binds to sequences in the activation domain of transcriptional activator Gcn4p, which contributes to the ability of Gcn4p to recruit the entire SWI/SNF complex to gene promoters (10). The requirement for Snf12p in assisting transcriptional activation appears to depend on both the activator protein and promoter involved (2, 11, 10). snf12 null mutants are viable, but temperature-sensitive, and also display defects in HO and HIS3 transcription, decreased structural integrity of the SWI/SNF complex, defective binding of the SWI/SNF complex to SNZ1 promoters, and hypersensitivity to 3-aminotriazole (3AT) (2, 11, 10, 11). Snf12p is similar to Rsc6p and human SMARCD2, and homologs are also present in Ashbya gossypii, Caenorhabditis elegans, and mice, indicating that Snf12p may belong to a family of related genes encoding proteins with analogous functions (2, 4, 12).

By regulating the structure of chromatin, chromatin remodeling complexes, all of which contain an ATPase as a central motor subunit, perform critical functions in the maintenance, transmission, and expression of eukaryotic genomes. The SWI/SNF chromatin remodeling complex is involved in DNA replication, stress response, and transcription, and binds DNA nonspecifically, altering nucleosome structure to facilitate binding of transcription factors. For some genes, transcriptional activators are able to target the SWI/SNF complex to upstream activation sequences (UAS) in the promoter. The SWI/SNF chromatin remodeling complex family contains two evolutionary conserved subclasses of chromatin remodeling factors, one subfamily includes yeast SWI/SNF, fly BAP, and mammalian BAF, and the other subfamily includes yeast RSC (Remodel the Structure of Chromatin), fly PBAP, and mammalian PBAF (4, 13, 14, 15, 16, 3, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 11, 27, 28, 10, 29, 30, 31, 32, 33, 28, 34, 35, 36, 37, 38).

It appears that some human SWI/SNF subunits act as tumor suppressors and there is also evidence that human SWI/SNF subunits are involved in controlling cell growth via their interaction with other tumor suppressors (39). Expression of adenovirus E1A oncoproteins, which are regulators of cellular and viral transcription, in Saccharomyces cerevisiae requires the function of the SWI/SNF complex, and expression of E1A in wild-type cells leads to a specific loss of SWI/SNF dependent transcription. These results suggest that the SWI/SNF complex is a target of these oncoproteins in mammalian cells and that the disruption of normal cell cycle control by E1A may be due in part to altered activity of the SWI/SNF complex (1).

Last updated: 2006-03-24 Contact SGD

References cited on this page View Complete Literature Guide for SNF12
1) Miller ME, et al.  (1996) Adenovirus E1A specifically blocks SWI/SNF-dependent transcriptional activation. Mol Cell Biol 16(10):5737-43
2) Cairns BR, et al.  (1996) Essential role of Swp73p in the function of yeast Swi/Snf complex. Genes Dev 10(17):2131-44
3) Cairns BR, et al.  (1994) A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast. Proc Natl Acad Sci U S A 91(5):1950-4
4) Cairns BR, et al.  (1996) RSC, an essential, abundant chromatin-remodeling complex. Cell 87(7):1249-60
5) Sudarsanam P and Winston F  (2000) The Swi/Snf family nucleosome-remodeling complexes and transcriptional control. Trends Genet 16(8):345-51
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
8) Carlson M, et al.  (1981) Mutants of yeast defective in sucrose utilization. Genetics 98(1):25-40
9) Smith CL, et al.  (2003) Structural analysis of the yeast SWI/SNF chromatin remodeling complex. Nat Struct Biol 10(2):141-5
10) Yoon S, et al.  (2003) Recruitment of SWI/SNF by Gcn4p does not require Snf2p or Gcn5p but depends strongly on SWI/SNF integrity, SRB mediator, and SAGA. Mol Cell Biol 23(23):8829-45
11) Natarajan K, et al.  (1999) Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator. Mol Cell 4(4):657-64
12) Treich I, et al.  (1998) Direct interaction between Rsc6 and Rsc8/Swh3,two proteins that are conserved in SWI/SNF-related complexes. Nucleic Acids Res 26(16):3739-45
13) Poch O and Winsor B  (1997) Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family. Yeast 13(11):1053-8
14) Peterson CL, et al.  (1998) Subunits of the yeast SWI/SNF complex are members of the actin-related protein (ARP) family. J Biol Chem 273(37):23641-4
15) Harata M, et al.  (2000) Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. J Biochem 128(4):665-71
16) Chervitz SA, et al.  (1998) Comparison of the complete protein sets of worm and yeast: orthology and divergence. Science 282(5396):2022-8
17) Cote J, et al.  (1994) Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex. Science 265(5168):53-60
18) Peterson CL, et al.  (1994) Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement. Proc Natl Acad Sci U S A 91(8):2905-8
19) Treich I, et al.  (1995) SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2. Mol Cell Biol 15(8):4240-8
20) Quinn J, et al.  (1996) DNA-binding properties of the yeast SWI/SNF complex. Nature 379(6568):844-7
21) Owen-Hughes T, et al.  (1996) Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex. Science 273(5274):513-6
22) Burns LG and Peterson CL  (1997) The yeast SWI-SNF complex facilitates binding of a transcriptional activator to nucleosomal sites in vivo. Mol Cell Biol 17(8):4811-9
23) Pollard KJ and Peterson CL  (1997) Role for ADA/GCN5 products in antagonizing chromatin-mediated transcriptional repression. Mol Cell Biol 17(11):6212-22
24) Utley RT, et al.  (1997) SWI/SNF stimulates the formation of disparate activator-nucleosome complexes but is partially redundant with cooperative binding. J Biol Chem 272(19):12642-9
25) Bazett-Jones DP, et al.  (1999) The SWI/SNF complex creates loop domains in DNA and polynucleosome arrays and can disrupt DNA-histone contacts within these domains. Mol Cell Biol 19(2):1470-8
26) Neely KE, et al.  (1999) Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays. Mol Cell 4(4):649-55
27) Steger DJ, et al.  (2003) Regulation of chromatin remodeling by inositol polyphosphates. Science 299(5603):114-6
28) Prochasson P, et al.  (2003) Targeting activity is required for SWI/SNF function in vivo and is accomplished through two partially redundant activator-interaction domains. Mol Cell 12(4):983-90
29) Lemieux K and Gaudreau L  (2004) Targeting of Swi/Snf to the yeast GAL1 UAS G requires the Mediator, TAF IIs, and RNA polymerase II. EMBO J 23(20):4040-50
30) Ferreira ME, et al.  (2005) Mechanism of transcription factor recruitment by acidic activators. J Biol Chem 280(23):21779-84
31) Whitehouse I, et al.  (1999) Nucleosome mobilization catalysed by the yeast SWI/SNF complex. Nature 400(6746):784-7
32) Yudkovsky N, et al.  (1999) Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators. Genes Dev 13(18):2369-74
33) Boyer LA, et al.  (2000) Roles of the histone H2A-H2B dimers and the (H3-H4)(2) tetramer in nucleosome remodeling by the SWI-SNF complex. J Biol Chem 275(16):11545-52
34) Logie C and Peterson CL  (1997) Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays. EMBO J 16(22):6772-82
35) Cote J, et al.  (1998) Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding. Proc Natl Acad Sci U S A 95(9):4947-52
36) Cosma MP, et al.  (1999) Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter. Cell 97(3):299-311
37) Flanagan JF and Peterson CL  (1999) A role for the yeast SWI/SNF complex in DNA replication. Nucleic Acids Res 27(9):2022-8
38) Ganster RW, et al.  (1998) Identification of a calcineurin-independent pathway required for sodium ion stress response in Saccharomyces cerevisiae. Genetics 150(1):31-42
39) Neely KE and Workman JL  (2002) The complexity of chromatin remodeling and its links to cancer. Biochim Biophys Acta 1603(1):19-29