SWI1/YPL016W Summary Help

Standard Name SWI1 1, 2, 3
Systematic Name YPL016W
Alias ADR6 4 , 5 , 6 , GAM3 7 , LPA1
Feature Type ORF, Verified
Description Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer (6, 8, 9, 10, 11, 12 and see Summary Paragraph)
Also known as: [SWI+] 11 , [SWI(+)]
Name Description SWItching deficient 1
Chromosomal Location
ChrXVI:521014 to 524958 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SWI1 GO evidence and references
  View Computational GO annotations for SWI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All SWI1 Phenotype evidence and references
Classical genetics
unspecified
Large-scale survey
null
reduction of function
Resources
interactions All SWI1 Interaction evidence and references
125 total interaction(s) for 68 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 50
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 4
  • Reconstituted Complex: 6
  • Two-hybrid: 15
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 10
  • Synthetic Rescue: 11
Resources
Expression Summary
histogram
Resources
Protein Information All SWI1 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:521014 to 524958 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3945 521014..524958 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005937
SUMMARY PARAGRAPH for SWI1

Swi1p is a component of the SWI/SNF complex (13, 9, 14, 15, 16), binds promoter activation domains (17, 18), and is required for transcription of a diverse set of genes, including HO and Ty retrotransposons (19, 20). Swi1p is also required for normal growth and initiation of meiosis (7).

swi1 null mutants are viable, but display sporulation defects, slow growth, decreased levels of Adh2p, and decreased heat-shock induction of HSP82 (6, 9, 21). swi1 mutants are also defective in mating-type switching, are highly sensitive to gamma-rays, and display increased rates of spontaneous oligomycin resistance resulting from mutations in the mitochondrial DNA (3, 22, 23, 1). swi1 mutations are supressed by spt2 mutations (24), and are synthetically lethal in combination with dst1 null mutations (25).

Swi1p is a member of a family of DNA-binding proteins that includes Drosophila melanogaster DRI, human retinol-binding proteins RBP1 and RBP2, human SMCX, which is associated with X-linked mental retardation (XLMR), and mouse Jumonji, which is required for neural tube formation (26). Swi1p is also similar to human ARID1A, and Hansenula polymorpha and Candida albicans Swi1p (27, 28, 29, 30). Swi1p also contains several regions which are similar to D. melanogaster Engrailed (13).

By regulating the structure of chromatin, chromatin remodeling complexes, all of which contain an ATPase as a central motor subunit, perform critical functions in the maintenance, transmission, and expression of eukaryotic genomes. The SWI/SNF chromatin remodeling complex is involved in DNA replication, stress response, and transcription, and binds DNA nonspecifically, altering nucleosome structure to facilitate binding of transcription factors. For some genes, transcriptional activators are able to target the SWI/SNF complex to upstream activation sequences (UAS) in the promoter. The SWI/SNF chromatin remodeling complex family contains two evolutionary conserved subclasses of chromatin remodeling factors, one subfamily includes yeast SWI/SNF, fly BAP, and mammalian BAF, and the other subfamily includes yeast RSC (Remodel the Structure of Chromatin), fly PBAP, and mammalian PBAF (31, 32, 8, 33, 34, 14, 35, 15, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 17, 46, 47, 48, 49, 50, 51, 17, 52, 53, 54, 55, 56).

It appears that some human SWI/SNF subunits act as tumor suppressors and there is also evidence that human SWI/SNF subunits are involved in controlling cell growth via their interaction with other tumor suppressors (57). Expression of adenovirus E1A oncoproteins, which are regulators of cellular and viral transcription, in Saccharomyces cerevisiae requires the function of the SWI/SNF complex, and expression of E1A in wild-type cells leads to a specific loss of SWI/SNF dependent transcription. These results suggest that the SWI/SNF complex is a target of these oncoproteins in mammalian cells and that the disruption of normal cell cycle control by E1A may be due in part to altered activity of the SWI/SNF complex (58).

Last updated: 2006-03-24

References cited on this page View Complete Literature Guide for SWI1
1) Stern M, et al.  (1984) Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol 178(4):853-68
2) Haber, J. and Rowe, L.  (1985) Personal Communication, Mortimer Map Edition 9
3) Haber JE and Garvik B  (1977) A new gene affecting the efficiency of mating-type interconversions in homothallic strains of Saccharomyces cerevisiae. Genetics 87(1):33-50
4) Yoshinaga SK, et al.  (1992) Roles of SWI1, SWI2, and SWI3 proteins for transcriptional enhancement by steroid receptors. Science 258(5088):1598-604
5) Taguchi AK and Young ET  (1987) The identification and characterization of ADR6, a gene required for sporulation and for expression of the alcohol dehydrogenase II isozyme from Saccharomyces cerevisiae. Genetics 116(4):523-30
6) Taguchi AK and Young ET  (1987) The cloning and mapping of ADR6, a gene required for sporulation and for expression of the alcohol dehydrogenase II isozyme from Saccharomyces cerevisiae. Genetics 116(4):531-40
7) Yoshimoto H, et al.  (1992) Identity of the GAM3 gene with ADR6, each required for transcription of the STA1 or ADH2 gene in Saccharomyces cerevisiae. Biosci Biotech Biochem 56:527-529
8) Peterson CL, et al.  (1998) Subunits of the yeast SWI/SNF complex are members of the actin-related protein (ARP) family. J Biol Chem 273(37):23641-4
9) Peterson CL and Herskowitz I  (1992) Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription. Cell 68(3):573-83
10) Burns LG and Peterson CL  (1997) Protein complexes for remodeling chromatin. Biochim Biophys Acta 1350(2):159-68
11) Du Z, et al.  (2008) Newly identified prion linked to the chromatin-remodeling factor Swi1 in Saccharomyces cerevisiae. Nat Genet 40(4):460-5
12) Mamo A, et al.  (2012) An integrated genomic approach identifies ARID1A as a candidate tumor-suppressor gene in breast cancer. Oncogene 31(16):2090-100
13) O'Hara PJ, et al.  (1988) The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain. Nucleic Acids Res 16(21):10153-69
14) Cairns BR, et al.  (1994) A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast. Proc Natl Acad Sci U S A 91(5):1950-4
15) Peterson CL, et al.  (1994) Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement. Proc Natl Acad Sci U S A 91(8):2905-8
16) Smith CL, et al.  (2003) Structural analysis of the yeast SWI/SNF chromatin remodeling complex. Nat Struct Biol 10(2):141-5
17) Prochasson P, et al.  (2003) Targeting activity is required for SWI/SNF function in vivo and is accomplished through two partially redundant activator-interaction domains. Mol Cell 12(4):983-90
18) Perez-Martin J and Johnson AD  (1998) The C-terminal domain of Sin1 interacts with the SWI-SNF complex in yeast. Mol Cell Biol 18(7):4157-64
19) Breeden L and Nasmyth K  (1987) Cell cycle control of the yeast HO gene: cis- and trans-acting regulators. Cell 48(3):389-97
20) Hirschhorn JN, et al.  (1992) Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure. Genes Dev 6(12A):2288-98
21) Zhao J, et al.  (2005) Domain-wide displacement of histones by activated heat shock factor occurs independently of Swi/Snf and is not correlated with RNA polymerase II density. Mol Cell Biol 25(20):8985-99
22) Foury F and Goffeau A  (1979) Genetic control of enhanced mutability of mitochondrial DNA and gamma-ray sensitivity in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(12):6529-33
23) Davidow LS and Haber JE  (1981) Relation between the efficiency of homothallic switching of yeast mating type genes and the distribution of cell types. Mol Cell Biol 1(12):1120-4
24) Sternberg PW, et al.  (1987) Activation of the yeast HO gene by release from multiple negative controls. Cell 48(4):567-77
25) Davie JK and Kane CM  (2000) Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex. Mol Cell Biol 20(16):5960-73
26) Gregory SL, et al.  (1996) Characterization of the dead ringer gene identifies a novel, highly conserved family of sequence-specific DNA-binding proteins. Mol Cell Biol 16(3):792-9
27) Dallas PB, et al.  (2000) The human SWI-SNF complex protein p270 is an ARID family member with non-sequence-specific DNA binding activity. Mol Cell Biol 20(9):3137-46
28) Nie Z, et al.  (2000) A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex. Mol Cell Biol 20(23):8879-88
29) Ozimek P, et al.  (2004) Hansenula polymorpha Swi1p and Snf2p are essential for methanol utilisation. FEMS Yeast Res 4(7):673-82
30) Li F and Palecek SP  (2005) Identification of Candida albicans genes that induce Saccharomyces cerevisiae cell adhesion and morphogenesis. Biotechnol Prog 21(6):1601-9
31) Cairns BR, et al.  (1996) RSC, an essential, abundant chromatin-remodeling complex. Cell 87(7):1249-60
32) Poch O and Winsor B  (1997) Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family. Yeast 13(11):1053-8
33) Harata M, et al.  (2000) Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus. J Biochem 128(4):665-71
34) Chervitz SA, et al.  (1998) Comparison of the complete protein sets of worm and yeast: orthology and divergence. Science 282(5396):2022-8
35) Cote J, et al.  (1994) Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex. Science 265(5168):53-60
36) Treich I, et al.  (1995) SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2. Mol Cell Biol 15(8):4240-8
37) Quinn J, et al.  (1996) DNA-binding properties of the yeast SWI/SNF complex. Nature 379(6568):844-7
38) Owen-Hughes T, et al.  (1996) Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex. Science 273(5274):513-6
39) Burns LG and Peterson CL  (1997) The yeast SWI-SNF complex facilitates binding of a transcriptional activator to nucleosomal sites in vivo. Mol Cell Biol 17(8):4811-9
40) Pollard KJ and Peterson CL  (1997) Role for ADA/GCN5 products in antagonizing chromatin-mediated transcriptional repression. Mol Cell Biol 17(11):6212-22
41) Utley RT, et al.  (1997) SWI/SNF stimulates the formation of disparate activator-nucleosome complexes but is partially redundant with cooperative binding. J Biol Chem 272(19):12642-9
42) Bazett-Jones DP, et al.  (1999) The SWI/SNF complex creates loop domains in DNA and polynucleosome arrays and can disrupt DNA-histone contacts within these domains. Mol Cell Biol 19(2):1470-8
43) Neely KE, et al.  (1999) Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays. Mol Cell 4(4):649-55
44) Natarajan K, et al.  (1999) Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator. Mol Cell 4(4):657-64
45) Steger DJ, et al.  (2003) Regulation of chromatin remodeling by inositol polyphosphates. Science 299(5603):114-6
46) Yoon S, et al.  (2003) Recruitment of SWI/SNF by Gcn4p does not require Snf2p or Gcn5p but depends strongly on SWI/SNF integrity, SRB mediator, and SAGA. Mol Cell Biol 23(23):8829-45
47) Lemieux K and Gaudreau L  (2004) Targeting of Swi/Snf to the yeast GAL1 UAS G requires the Mediator, TAF IIs, and RNA polymerase II. EMBO J 23(20):4040-50
48) Ferreira ME, et al.  (2005) Mechanism of transcription factor recruitment by acidic activators. J Biol Chem 280(23):21779-84
49) Whitehouse I, et al.  (1999) Nucleosome mobilization catalysed by the yeast SWI/SNF complex. Nature 400(6746):784-7
50) Yudkovsky N, et al.  (1999) Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators. Genes Dev 13(18):2369-74
51) Boyer LA, et al.  (2000) Roles of the histone H2A-H2B dimers and the (H3-H4)(2) tetramer in nucleosome remodeling by the SWI-SNF complex. J Biol Chem 275(16):11545-52
52) Logie C and Peterson CL  (1997) Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays. EMBO J 16(22):6772-82
53) Cote J, et al.  (1998) Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding. Proc Natl Acad Sci U S A 95(9):4947-52
54) Cosma MP, et al.  (1999) Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter. Cell 97(3):299-311
55) Flanagan JF and Peterson CL  (1999) A role for the yeast SWI/SNF complex in DNA replication. Nucleic Acids Res 27(9):2022-8
56) Ganster RW, et al.  (1998) Identification of a calcineurin-independent pathway required for sodium ion stress response in Saccharomyces cerevisiae. Genetics 150(1):31-42
57) Neely KE and Workman JL  (2002) The complexity of chromatin remodeling and its links to cancer. Biochim Biophys Acta 1603(1):19-29
58) Miller ME, et al.  (1996) Adenovirus E1A specifically blocks SWI/SNF-dependent transcriptional activation. Mol Cell Biol 16(10):5737-43