SWA2/YDR320C Summary Help

Standard Name SWA2 1
Systematic Name YDR320C
Alias AUX1 , BUD24 2
Feature Type ORF, Verified
Description Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles (1, 3 and see Summary Paragraph)
Name Description Synthetic lethal With Arf1 1
Chromosomal Location
ChrIV:1108101 to 1106095 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SWA2 GO evidence and references
  View Computational GO annotations for SWA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
290 total interaction(s) for 172 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 21
  • Affinity Capture-Western: 3
  • Co-crystal Structure: 1
  • PCA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 193
  • Phenotypic Suppression: 1
  • Positive Genetic: 38
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 11
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 668
Molecular Weight (Da) 75,019
Isoelectric Point (pI) 5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1108101 to 1106095 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2007 1108101..1106095 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002728
SUMMARY PARAGRAPH for SWA2

Hsp40/DnaJ is a family of proteins, established by bacterial DnaJ, that regulates Hsp70 chaperone activity. Hsp40s stimulate the intrinsically weak ATPase activity of Hsp70 proteins and facilitate Hsp70 interaction with polypeptide substrates. Hsp70 family members often have multiple Hsp40 partners, and these specific pairings govern Hsp70 chaperone involvement in particular processes (reviewed in 4, 5, and 6). All Hsp40s contain a highly conserved 75-amino acid J domain, which interacts with the ATPase domain of Hsp70 to stimulate ATP hydrolysis. However, there are also other conserved structural domains, and based on the presence or absence of these regions, the Hsp40 family can be divided into three subtypes: type I, type II and type III (a comprehensive overview of the structural features of the different HSP40 subtypes can be found in 6). Sequence analysis of the S. cerevisiae genome has revealed 22 proteins in the Hsp40/DnaJ family: YDJ1, XDJ1, APJ1, SIS1, DJP1, ZUO1, SWA2, JJJ1, JJJ2, JJJ3, CAJ1, CWC23, MDJ1, MDJ2, PAM18, JAC1, JID1, SCJ1, HLJ1, JEM1, SEC63, and ERJ5 (6).

Last updated: 2006-12-19 Contact SGD

References cited on this page View Complete Literature Guide for SWA2
1) Gall WE, et al.  (2000) The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast. Curr Biol 10(21):1349-58
2) Ni L and Snyder M  (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
3) Pishvaee B, et al.  (2000) A yeast DNA J protein required for uncoating of clathrin-coated vesicles in vivo. Nat Cell Biol 2(12):958-63
4) Qiu XB, et al.  (2006) The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones. Cell Mol Life Sci 63(22):2560-2570
5) Cyr DM, et al.  (1994) DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70. Trends Biochem Sci 19(4):176-81
6) Walsh P, et al.  (2004) The J-protein family: modulating protein assembly, disassembly and translocation. EMBO Rep 5(6):567-71