SUP45/YBR143C Summary Help

Standard Name SUP45
Systematic Name YBR143C
Alias SAL4 , SUP1 , SUP47
Feature Type ORF, Verified
Description Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p (1, 2, 3, 4, 5, 6)
Name Description SUPpressor
Gene Product Alias eRF1
Chromosomal Location
ChrII:532182 to 530869 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 81 cM
Gene Ontology Annotations All SUP45 GO evidence and references
  View Computational GO annotations for SUP45
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
109 total interaction(s) for 60 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 56
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 11
  • Biochemical Activity: 2
  • Co-fractionation: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Rescue: 10
  • Negative Genetic: 3
  • Phenotypic Enhancement: 3
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 437
Molecular Weight (Da) 49,005
Isoelectric Point (pI) 4.77
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrII:532182 to 530869 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 81 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1314 532182..530869 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000347
References cited on this page View Complete Literature Guide for SUP45
1) Stansfield I, et al.  (1992) Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination. Mol Microbiol 6(23):3469-78
2) Himmelfarb HJ, et al.  (1985) Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product. Mol Cell Biol 5(4):816-22
3) Stansfield I, et al.  (1995) The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J 14(17):4365-73
4) Valouev IA, et al.  (2004) Translation termination factors function outside of translation: yeast eRF1 interacts with myosin light chain, Mlc1p, to effect cytokinesis. Mol Microbiol 53(2):687-96
5) Heurgue-Hamard V, et al.  (2006) The Zinc Finger Protein Ynr046w Is Plurifunctional and a Component of the eRF1 Methyltransferase in Yeast. J Biol Chem 281(47):36140-8
6) Studte P, et al.  (2008) tRNA and protein methylase complexes mediate zymocin toxicity in yeast. Mol Microbiol 69(5):1266-77