SUB2/YDL084W Summary

SUB2 BASIC INFORMATION

Standard Name	SUB2
Systematic Name	YDL084W
Feature Type	ORF, Verified
Description	Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56 (1, 2, 3, 4)
Name Description	SUppressor of Brr1-1

GO Annotations	All SUB2 GO evidence and references
	View Computational GO annotations for SUB2
Molecular Function
Manually curated	ATP-dependent RNA helicase activity (IGI, ISA)
Biological Process
Manually curated	chromatin silencing at telomere (IMP) mRNA 3'-end processing (IMP) mRNA export from nucleus (IDA, IMP, IPI) nuclear mRNA branch site recognition (TAS) nuclear mRNA splicing, via spliceosome (IMP) RNA elongation from RNA polymerase II promoter (IMP) transcription-coupled nucleotide-excision repair (IMP)
Cellular Component
Manually curated	chromosome, telomeric region (IPI) spliceosomal complex (TAS) transcription export complex (IPI)
High-throughput	nucleus (IDA)

Mutant Phenotype	All SUB2 Phenotype details and references
Classical genetics
null	mRNAs accumulation: decreased inviable mitotic recombination: increased vegetative growth: decreased
reduction of function	growth in exponential phase: decreased rate
repressible	unspliced pre-RNA accumulation: decreased
Large-scale survey
null	inviable
overexpression	vegetative growth: decreased

Interactions	SUB2 All interactions details and references
	144 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 38 Affinity Capture-RNA: 1 Affinity Capture-Western: 19 Co-purification: 1 Reconstituted Complex: 4 Two-hybrid: 9
Genetic Interactions	Dosage Growth Defect: 14 Dosage Rescue: 9 Phenotypic Enhancement: 25 Phenotypic Suppression: 2 Synthetic Growth Defect: 2 Synthetic Lethality: 15 Synthetic Rescue: 5

Sequence Information

ChrIV:305237 to 306577 | |

Last Update

Coordinates: 2004-02-11 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1341	305237..306577	2004-02-11	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002242

SUB2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SUB2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SUB2]

1)	Fan HY, et al. (2001) High-copy-number expression of Sub2p, a member of the RNA helicase superfamily, suppresses hpr1-mediated genomic instability. Mol Cell Biol 21(16):5459-70
2)	Jensen TH, et al. (2001) The DECD box putative ATPase Sub2p is an early mRNA export factor. Curr Biol 11(21):1711-5
3)	Strasser K and Hurt E (2001) Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p. Nature 413(6856):648-52
4)	Strasser K, et al. (2002) TREX is a conserved complex coupling transcription with messenger RNA export. Nature 417(6886):304-8

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