STP22/YCL008C Summary Help

Standard Name STP22 1
Systematic Name YCL008C
Alias VPS23 2 , 3 , VPL15 3 , AGS1 4
Feature Type ORF, Verified
Description Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype; (5, 6, 7)
Name Description STerile Pseudoreversion 1
Chromosomal Location
ChrIII:106853 to 105696 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All STP22 GO evidence and references
  View Computational GO annotations for STP22
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
198 total interaction(s) for 150 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Biochemical Activity: 1
  • Co-crystal Structure: 2
  • Co-fractionation: 3
  • Co-purification: 2
  • PCA: 4
  • Reconstituted Complex: 2
  • Two-hybrid: 47

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 15
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 34
  • Positive Genetic: 3
  • Synthetic Growth Defect: 39
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 10
  • Synthetic Rescue: 8

Expression Summary
Length (a.a.) 385
Molecular Weight (Da) 43,330
Isoelectric Point (pI) 5.53
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIII:106853 to 105696 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2006-01-12 | Sequence: 2004-02-18
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1158 106853..105696 2006-01-12 2004-02-18
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000514
References cited on this page View Complete Literature Guide for STP22
1) Li Y, et al.  (1999) Yeast mutants affecting possible quality control of plasma membrane proteins. Mol Cell Biol 19(5):3588-99
2) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
3) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
4) Wickert S, et al.  (1998) A small protein (Ags1p) and the Pho80p-Pho85p kinase complex contribute to aminoglycoside antibiotic resistance of the yeast Saccharomyces cerevisiae. J Bacteriol 180(7):1887-94
5) Babst M, et al.  (2000) Mammalian tumor susceptibility gene 101 (TSG101) and the yeast homologue, Vps23p, both function in late endosomal trafficking. Traffic 1(3):248-58
6) Katzmann DJ, et al.  (2001) Ubiquitin-dependent sorting into the multivesicular body pathway requires the function of a conserved endosomal protein sorting complex, ESCRT-I. Cell 106(2):145-55
7) Herrador A, et al.  (2013) A Mechanism for Protein Monoubiquitination Dependent on a trans-Acting Ubiquitin-binding Domain. J Biol Chem 288(23):16206-11