STE6/YKL209C Summary Help

Standard Name STE6
Systematic Name YKL209C
Feature Type ORF, Verified
Description Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description STErile
Chromosomal Location
ChrXI:46295 to 42423 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -152.3 cM
Gene Ontology Annotations All STE6 GO evidence and references
  View Computational GO annotations for STE6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Classical genetics
Large-scale survey
33 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,290
Molecular Weight (Da) 144,764
Isoelectric Point (pI) 7.91
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:46295 to 42423 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -152.3 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3873 46295..42423 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000001692

STE6 was first identified as a gene necessary for mating in MAT a cells (6, 7). The Ste6 protein is a membrane transporter of the ABC (ATP-binding cassette) family. While alpha factor is exported in MATalpha cells via the classical secretion pathway, a-factor is pumped out of the cell by the MATa cell-specific protein Ste6p (3, 1). In MATalpha cells, STE6 expression is repressed by the Alpha2 gene product (7, 8). Ste6p is related to human MDR1, a P-glycoprotein/multiple drug resistance protein (1, 3); several MDR proteins, including mouse Mdr3 and cystic fibrosis transmembrane conductance regulator (CFTR)/Ste6 chimeras, can complement ste6 null mutations in vivo in yeast (9, 10). In fact, a model has been established by these cross-species complementation tests to study the function of higher eukaryotic MDR proteins (11).

Last updated: 1999-03-31 Contact SGD

References cited on this page View Complete Literature Guide for STE6
1) McGrath JP and Varshavsky A  (1989) The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein. Nature 340(6232):400-4
2) Michaelis S  (1993) STE6, the yeast a-factor transporter. Semin Cell Biol 4(1):17-27
3) Kuchler K, et al.  (1989) Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells. EMBO J 8(13):3973-84
4) Loayza D and Michaelis S  (1998) Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the a-factor transporter in Saccharomyces cerevisiae. Mol Cell Biol 18(2):779-89
5) Ketchum CJ, et al.  (2001) The yeast a-factor transporter Ste6p, a member of the ABC superfamily, couples ATP hydrolysis to pheromone export. J Biol Chem 276(31):29007-11
6) Chan RK, et al.  (1983) Extracellular suppression allows mating by pheromone-deficient sterile mutants of Saccharomyces cerevisiae. J Bacteriol 155(2):903-6
7) Wilson KL and Herskowitz I  (1984) Negative regulation of STE6 gene expression by the alpha 2 product of Saccharomyces cerevisiae. Mol Cell Biol 4(11):2420-7
8) Wilson KL and Herskowitz I  (1986) Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 83(8):2536-40
9) Raymond M, et al.  (1992) Functional complementation of yeast ste6 by a mammalian multidrug resistance mdr gene. Science 256(5054):232-4
10) Teem JL, et al.  (1993) Identification of revertants for the cystic fibrosis delta F508 mutation using STE6-CFTR chimeras in yeast. Cell 73(2):335-46
11) Hemenway CS and Heitman J  (1996) Immunosuppressant target protein FKBP12 is required for P-glycoprotein function in yeast. J Biol Chem 271(31):18527-34