SSN3/YPL042C Summary Help

Standard Name SSN3 1
Systematic Name YPL042C
Alias GIG2 2 , NUT7 3 , SRB10 4 , UME5 5 , RYE5 , CDK8 6 , SSX7 7 , URR1 8
Feature Type ORF, Verified
Description Cyclin-dependent protein kinase; component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression (2, 4, 9)
Name Description Suppressor of SNf1 1
Chromosomal Location
ChrXVI:474707 to 473040 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SSN3 GO evidence and references
  View Computational GO annotations for SSN3
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Targets 11 genes
Regulators 1 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
390 total interaction(s) for 263 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 51
  • Affinity Capture-Western: 26
  • Biochemical Activity: 22
  • Co-crystal Structure: 1
  • Co-purification: 2
  • PCA: 3
  • Reconstituted Complex: 5
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 3
  • Negative Genetic: 106
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 8
  • Positive Genetic: 106
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 6
  • Synthetic Rescue: 10

Resources
Expression Summary
histogram
Resources
Length (a.a.) 555
Molecular Weight (Da) 62,847
Isoelectric Point (pI) 10.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:474707 to 473040 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1668 474707..473040 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005963
References cited on this page View Complete Literature Guide for SSN3
1) Carlson M, et al.  (1984) A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast. Genetics 107(1):19-32
2) Balciunas D and Ronne H  (1995) Three subunits of the RNA polymerase II mediator complex are involved in glucose repression. Nucleic Acids Res 23(21):4421-5
3) Tabtiang RK and Herskowitz I  (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
4) Liao SM, et al.  (1995) A kinase-cyclin pair in the RNA polymerase II holoenzyme. Nature 374(6518):193-6
5) Surosky RT, et al.  (1994) The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose. Mol Cell Biol 14(5):3446-58
6) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
7) Li L, et al.  (2005) Genetic interactions between mediator and the late G1-specific transcription factor Swi6 in Saccharomyces cerevisiae. Genetics 171(2):477-88
8) Becker P and Johnston M  (2014) personal communication
9) Kuchin S, et al.  (1995) Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast. Proc Natl Acad Sci U S A 92(9):4006-10