SSN2/YDR443C Summary Help

Standard Name SSN2 1
Systematic Name YDR443C
Alias NUT8 2 , SCA1 , SRB9 , UME2 , RYE3 , MED13 3 , SSX5 4
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation (1, 5, 6)
Name Description Suppressor of SNf1 1
Chromosomal Location
ChrIV:1349938 to 1345676 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SSN2 GO evidence and references
  View Computational GO annotations for SSN2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 27 genes
Regulators 2 genes
Classical genetics
Large-scale survey
264 total interaction(s) for 188 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 49
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 10
  • Biochemical Activity: 2
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 101
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 5
  • Positive Genetic: 44
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 8
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,420
Molecular Weight (Da) 159,999
Isoelectric Point (pI) 5.57
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1349938 to 1345676 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4263 1349938..1345676 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002851
References cited on this page View Complete Literature Guide for SSN2
1) Carlson M, et al.  (1984) A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast. Genetics 107(1):19-32
2) Tabtiang RK and Herskowitz I  (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
3) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
4) Li L, et al.  (2005) Genetic interactions between mediator and the late G1-specific transcription factor Swi6 in Saccharomyces cerevisiae. Genetics 171(2):477-88
5) Song W, et al.  (1996) SSN genes that affect transcriptional repression in Saccharomyces cerevisiae encode SIN4, ROX3, and SRB proteins associated with RNA polymerase II. Mol Cell Biol 16(1):115-20
6) Larschan E and Winston F  (2005) The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA complex during Gal4-activated transcription. Mol Cell Biol 25(1):114-23