SRV2/YNL138W Summary Help

Standard Name SRV2 1
Systematic Name YNL138W
Alias CAP
Feature Type ORF, Verified
Description CAP (cyclase-associated protein); N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus, in physically and genetically separate activity, binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis (1, 2, 3, 4)
Name Description Suppressor of RasVal19 1
Chromosomal Location
ChrXIV:366741 to 368321 | ORF Map | GBrowse
Gbrowse
Genetic position: -101 cM
Gene Ontology Annotations All SRV2 GO evidence and references
  View Computational GO annotations for SRV2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
conditional
null
unspecified
Large-scale survey
conditional
null
overexpression
Resources
115 total interaction(s) for 68 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Co-fractionation: 3
  • PCA: 13
  • Protein-peptide: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 2
  • Negative Genetic: 3
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 11
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 526
Molecular Weight (Da) 57,521
Isoelectric Point (pI) 5.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:366741 to 368321 | ORF Map | GBrowse
SGD ORF map
Genetic position: -101 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1581 366741..368321 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005082
References cited on this page View Complete Literature Guide for SRV2
1) Fedor-Chaiken M, et al.  (1990) SRV2, a gene required for RAS activation of adenylate cyclase in yeast. Cell 61(2):329-40
2) Freeman NL, et al.  (1995) An actin monomer binding activity localizes to the carboxyl-terminal half of the Saccharomyces cerevisiae cyclase-associated protein. J Biol Chem 270(10):5680-5
3) Mattila PK, et al.  (2004) A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein. Mol Biol Cell 15(11):5158-71
4) Chaudhry F, et al.  (2013) Srv2/cyclase-associated protein forms hexameric shurikens that directly catalyze actin filament severing by cofilin. Mol Biol Cell 24(1):31-41