SRT1/YMR101C Summary Help

Standard Name SRT1
Systematic Name YMR101C
Feature Type ORF, Verified
Description Cis-prenyltransferase; involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase (1, 2, 3 and see Summary Paragraph)
Chromosomal Location
ChrXIII:469476 to 468445 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SRT1 GO evidence and references
  View Computational GO annotations for SRT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Large-scale survey
null
overexpression
Resources
9 total interaction(s) for 6 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 343
Molecular Weight (Da) 40,200
Isoelectric Point (pI) 10.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:469476 to 468445 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1032 469476..468445 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004707
SUMMARY PARAGRAPH for SRT1

Dolichols are a class of polyisoprenoid alcohols with five or more isoprene units in which the C2-C3 bond is saturated. In yeast, dolichols with 14-18 isoprene units are the carrier molecules on which many of the steps in N-linked glycosylation, O-linked glycosylation, and GPI anchor synthesis occur on the membrane of the endoplasmic reticulum (ER) (reviewed in 4). In mammals, dolichols with 19-22 isoprene units are the carrier molecules on which these steps occur.

SRT1 encodes a cis-prenyltransferase that synthesizes the dolichol precursor dehydrodolichyl diphosphate with 19-22 isoprenoid units (3). It was originally identified as a multicopy suppressor of an rer2 mutant (1, 3), which lacks a homologous cis-prenyltransferase that synthesizes dolichol precursors with 14-18 isoprene units. At a low frequency, rer2 deletion mutants revert to near-wild-type growth rates and glycosylation levels but make only the longer chain dolichols, which are detectable only in the absence of Rer2p (2, 3). Unlike Rer2p, which is found primarily in the ER, Srt1p is found on lipid particles (3). Srt1 mutants grow normally (1, 2), have normal gylcosylation (2), and are not sensitive to hygromycin B (1). Deletion of both SRT1 and RER2 is lethal (1). Unlike RER2, SRT1 is induced in the stationary phase (3). Overexpression of Erg20p, which synthesizes the Rer2p substrate farnesyl diphosphate, induces expression of SRT1, RER2, and DPM1.

Last updated: 2005-07-08 Contact SGD

References cited on this page View Complete Literature Guide for SRT1
1) Sato M, et al.  (1999) The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis. Mol Cell Biol 19(1):471-83
2) Schenk B, et al.  (2001) An alternative cis-isoprenyltransferase activity in yeast that produces polyisoprenols with chain lengths similar to mammalian dolichols. Glycobiology 11(1):89-98
3) Sato M, et al.  (2001) Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. Genes Cells 6(6):495-506
4) Grabinska K and Palamarczyk G  (2002) Dolichol biosynthesis in the yeast Saccharomyces cerevisiae: an insight into the regulatory role of farnesyl diphosphate synthase. FEMS Yeast Res 2(3):259-65