SRB8/YCR081W Summary

SRB8/YCR081W Summary

Standard Name	SRB8 1
Systematic Name	YCR081W
Alias	GIG1 2 , NUT6 3 , SSN5 , YCR080W , MED12 4
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression (1, 2, 5)
Name Description	Suppressor of RNA polymerase B 1

Chromosomal Location	ChrIII:254371 to 258654 \| ORF Map \| GBrowse

Gene Ontology Annotations All SRB8 GO evidence and references

	View Computational GO annotations for SRB8
Molecular Function
Manually curated	RNA polymerase II transcription coactivator activity (IDA, IMP)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription by galactose (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	mediator complex (IDA)

Mutant phenotypes All SRB8 Phenotype evidence and references

Classical genetics
null	metal resistance: increased metal resistance: decreased Ptr2p-GFP accumulation: increased utilization of carbon source: decreased vegetative growth: decreased
Large-scale survey
null	cell size: increased polyphosphate accumulation: abnormal glycogen accumulation: increased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased nutrient utilization: increased resistance to sirolimus: increased resistance to hydroxyurea: increased resistance to wortmannin: decreased resistance to amiodarone: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to mycophenolic acid: decreased resistance to tunicamycin: decreased resistance to mefloquine: decreased resistance to sodium selenide: decreased resistance to cadmium dichloride: decreased resistance to cycloheximide: decreased respiratory growth: decreased rate sporulation efficiency: decreased stress resistance: decreased telomere length: increased toxin resistance: decreased transposable element transposition: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SRB8 Interaction evidence and references

	191 total interaction(s) for 143 unique genes/features.
Physical Interactions	Affinity Capture-MS: 45 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Co-purification: 1 PCA: 1 Two-hybrid: 1
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 56 Phenotypic Enhancement: 3 Phenotypic Suppression: 35 Positive Genetic: 20 Synthetic Growth Defect: 12 Synthetic Lethality: 3 Synthetic Rescue: 6
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SRB8 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIII:254371 to 258654 | |

This feature contains embedded feature(s): YCR081C-A

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 2000-09-13

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4284	254371..258654	2011-02-03	2000-09-13

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000677

References cited on this page View Complete Literature Guide for SRB8

1)	Hengartner CJ, et al. (1995) Association of an activator with an RNA polymerase II holoenzyme. Genes Dev 9(8):897-910
2)	Balciunas D and Ronne H (1995) Three subunits of the RNA polymerase II mediator complex are involved in glucose repression. Nucleic Acids Res 23(21):4421-5
3)	Tabtiang RK and Herskowitz I (1998) Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae. Mol Cell Biol 18(8):4707-18
4)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
5)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9