SRB6/YBR253W Summary Help

Standard Name SRB6 1
Systematic Name YBR253W
Alias MED22 2
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation (1, 3, 4)
Name Description Suppressor of RNA polymerase B 1
Chromosomal Location
ChrII:723270 to 723635 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SRB6 GO evidence and references
  View Computational GO annotations for SRB6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 100 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
repressible
unspecified
Resources
162 total interaction(s) for 55 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 109
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 18
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 8
  • PCA: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 121
Molecular Weight (Da) 13,863
Isoelectric Point (pI) 4.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:723270 to 723635 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..366 723270..723635 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000457
References cited on this page View Complete Literature Guide for SRB6
1) Thompson CM, et al.  (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
4) Takagi Y, et al.  (2006) Head module control of mediator interactions. Mol Cell 23(3):355-64