Standard Name	SRB5 1
Systematic Name	YGR104C
Alias	MED18 2
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance (1, 3, 4, 5, 6)
Name Description	Suppressor of RNA polymerase B 1

Chromosomal Location	ChrVII:698368 to 697445 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for SRB5
Molecular Function
Manually curated	core RNA polymerase II binding transcription factor activity (IDA, IGI) RNA polymerase II transcription factor recruiting transcription factor activity (IMP) TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly (IDA)
Biological Process
Manually curated	positive regulation of transcription from RNA polymerase II promoter (IDA) RNA polymerase II transcriptional preinitiation complex assembly (IDA) transcriptional open complex formation at RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	core mediator complex (IDA)

Classical genetics
null	anaerobic growth: decreased rate auxotrophy cold sensitivity: increased heat sensitivity: increased resistance to caffeine: normal resistance to formamide: decreased resistance to nikkomycin Z: decreased resistance to caffeine: decreased resistance to hygromycin B: decreased resistance to trisodium vanadate: decreased resistance to magnesium dichloride: decreased resistance to lithium chloride: decreased resistance to cycloheximide: decreased resistance to hydroxyurea: decreased resistance to thiabendazole: decreased respiratory growth: decreased rate subcellular morphology: abnormal vegetative growth: decreased
Large-scale survey
null	acid pH resistance: decreased alkaline pH resistance: decreased bud morphology: abnormal cell size: increased glycogen accumulation: decreased proton accumulation: increased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased resistance to hygromycin B: decreased resistance to quinine: decreased resistance to ethanol: decreased resistance to CTBT: decreased resistance to methyl methanesulfonate: decreased resistance to acetaldehyde: decreased resistance to hydroxyurea: decreased resistance to oleate: decreased resistance to spermine: decreased resistance to boric acid: decreased resistance to dimethyl sulfoxide: decreased resistance to lovastatin: decreased resistance to L-1,4-dithiothreitol: decreased resistance to doxorubicin: decreased resistance to acrolein: decreased resistance to glycolaldehyde: decreased resistance to sorbate: decreased telomere length: decreased toxin resistance: decreased transposable element transposition: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	328 total interaction(s) for 90 unique genes/features.
Physical Interactions	Affinity Capture-MS: 142 Affinity Capture-Western: 102 Co-crystal Structure: 2 Co-localization: 1 Co-purification: 36 PCA: 2 Reconstituted Complex: 11 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 2 Synthetic Growth Defect: 9 Synthetic Lethality: 17 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrVII:698368 to 697445 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..924 698368..697445 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003336