SQS1/YNL224C Summary Help

Standard Name SQS1 1
Systematic Name YNL224C
Alias PFA1 2
Feature Type ORF, Verified
Description Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress (1, 2, 3, 4, 5)
Name Description SQuelch of Splicing suppression 1
Chromosomal Location
ChrXIV:227099 to 224796 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SQS1 GO evidence and references
  View Computational GO annotations for SQS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
79 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 2
  • Negative Genetic: 26
  • Phenotypic Enhancement: 1
  • Positive Genetic: 12
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 767
Molecular Weight (Da) 86,949
Isoelectric Point (pI) 5.48
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:227099 to 224796 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2304 227099..224796 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005168
References cited on this page View Complete Literature Guide for SQS1
1) Pandit S, et al.  (2006) Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A 103(37):13700-5
2) Lebaron S, et al.  (2005) The splicing ATPase prp43p is a component of multiple preribosomal particles. Mol Cell Biol 25(21):9269-82
3) Pertschy B, et al.  (2009) RNA helicase Prp43 and its co-factor Pfa1 promote 20 to 18 S rRNA processing catalyzed by the endonuclease Nob1. J Biol Chem 284(50):35079-91
4) Lebaron S, et al.  (2009) The ATPase and helicase activities of Prp43p are stimulated by the G-patch protein Pfa1p during yeast ribosome biogenesis. EMBO J 28(24):3808-19
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76