Standard Name	SPT23
Systematic Name	YKL020C
Feature Type	ORF, Verified
Description	ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication (1, 2, 3)
Name Description	SuPpressor of Ty

Chromosomal Location	ChrXI:402080 to 398832 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

Molecular Function
High-throughput	DNA binding (IPI)
Biological Process
Manually curated	cellular response to cold (IGI) chromatin silencing at silent mating-type cassette (IGI) positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of unsaturated fatty acid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter (IGI)
Cellular Component
Manually curated	integral to endoplasmic reticulum membrane (IDA, ISM) nucleus (IDA)

Binding motifs	Predicted SPT23 Binding Site Locations
	RAAATSAA 4 [Search intergenic regions for RAAATSAA]
Resources	ScerTF | YeTFaSCo

Classical genetics
overexpression	budding: abnormal cell size: abnormal cellular morphology: abnormal
Large-scale survey
null	competitive fitness: increased haploproficient resistance to PM02734: increased resistance to bleomycin: increased resistance to cycloheximide: increased resistance to fenpropimorph: increased resistance to 4-chlorophenol: decreased resistance to mycophenolic acid: decreased resistance to camptothecin: decreased viable
overexpression	vegetative growth: decreased vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	135 total interaction(s) for 80 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 1 Affinity Capture-Western: 11 Biochemical Activity: 3 PCA: 1 Reconstituted Complex: 2 Two-hybrid: 3
Genetic Interactions	Dosage Growth Defect: 2 Dosage Lethality: 1 Dosage Rescue: 3 Negative Genetic: 69 Phenotypic Enhancement: 3 Phenotypic Suppression: 3 Positive Genetic: 19 Synthetic Lethality: 6 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:402080 to 398832 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3249 402080..398832 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001503