SPT21/YMR179W Summary Help

Standard Name SPT21 1, 2
Systematic Name YMR179W
Feature Type ORF, Verified
Description Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress (1, 3, 4, 5, 6)
Name Description SuPpressor of Ty 7
Chromosomal Location
ChrXIII:619858 to 622134 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPT21 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 8 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
231 total interaction(s) for 186 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 3
  • Protein-RNA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 118
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 7
  • Positive Genetic: 62
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 7
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 758
Molecular Weight (Da) 84,696
Isoelectric Point (pI) 5.88
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:619858 to 622134 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2277 619858..622134 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004791
References cited on this page View Complete Literature Guide for SPT21
1) Natsoulis G, et al.  (1994) The SPT10 and SPT21 genes of Saccharomyces cerevisiae. Genetics 136(1):93-105
2) Boeke, J.D.  (1992) Personal Communication, Mortimer Map Edition 11
3) Dollard C, et al.  (1994) SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. Mol Cell Biol 14(8):5223-8
4) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
5) Chang JS and Winston F  (2011) Spt10 and Spt21 Are Required for Transcriptional Silencing in Saccharomyces cerevisiae. Eukaryot Cell 10(1):118-29
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Fassler JS and Winston F  (1988) Isolation and analysis of a novel class of suppressor of Ty insertion mutations in Saccharomyces cerevisiae. Genetics 118(2):203-12