Standard Name	SPT20
Systematic Name	YOL148C
Alias	ADA5
Feature Type	ORF, Verified
Description	Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay (1, 2)
Name Description	SuPpressor of Ty

Chromosomal Location	ChrXV:47574 to 45760 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for SPT20
Molecular Function
Manually curated	transcription cofactor activity (IGI, IMP, IPI)
Biological Process
Manually curated	chromatin modification (IDA) histone acetylation (IDA) UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response (IMP)
Cellular Component
Manually curated	SAGA complex (IDA) SLIK (SAGA-like) complex (IDA)

Classical genetics
null	X ray resistance: increased chronological lifespan: increased osmotic stress resistance: decreased oxidative stress resistance: decreased resistance to mycophenolic acid: decreased resistance to 6-azauracil: decreased
Large-scale survey
null	acid pH resistance: decreased alkaline pH resistance: decreased bud morphology: abnormal glycogen accumulation: decreased dessication resistance: decreased freeze-thaw resistance: decreased haploinsufficient metal resistance: decreased resistance to mycophenolic acid: decreased resistance to wortmannin: decreased resistance to sirolimus: decreased resistance to cycloheximide: decreased resistance to paromomycin: decreased resistance to cadmium dichloride: decreased resistance to farnesol: decreased resistance to CTBT: decreased resistance to tirapazamine: decreased resistance to camptothecin: decreased resistance to hydroxyurea: decreased resistance to fluconazole: decreased resistance to sulfometuron methyl: decreased resistance to tunicamycin: decreased resistance to Echinacea extract: decreased resistance to hydrogen chloride: decreased resistance to 1,4-dithiothreitol: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to boric acid: decreased resistance to ethanol: decreased resistance to calcium dichloride: decreased resistance to doxorubicin: decreased resistance to glycolaldehyde: decreased resistance to sorbate: decreased respiratory growth: absent sporulation: absent transposable element transposition: decreased utilization of carbon source: decreased vegetative growth: decreased rate vegetative growth: absent
overexpression	silencing: abnormal
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	233 total interaction(s) for 83 unique genes/features.
Physical Interactions	Affinity Capture-MS: 143 Affinity Capture-RNA: 1 Affinity Capture-Western: 23 Biochemical Activity: 3 Co-fractionation: 1 Co-localization: 3 Co-purification: 7 Reconstituted Complex: 8 Two-hybrid: 1
Genetic Interactions	Phenotypic Enhancement: 3 Synthetic Growth Defect: 9 Synthetic Lethality: 24 Synthetic Rescue: 7
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:47574 to 45760 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1815 47574..45760 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005508