SPT10/YJL127C Summary Help

Standard Name SPT10 1
Systematic Name YJL127C
Alias CRE1 2 , SUD1 3
Feature Type ORF, Verified
Description Putative histone acetylase with a role in transcriptional silencing; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box (3, 4, 5, 6 and see Summary Paragraph)
Name Description SuPpressor of Ty 7
Chromosomal Location
ChrX:184220 to 182298 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -87 cM
Gene Ontology Annotations All SPT10 GO evidence and references
  View Computational GO annotations for SPT10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Targets 1177 genes
Regulators 2 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
overexpression
Resources
122 total interaction(s) for 102 unique genes/features.
Physical Interactions
  • Co-localization: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 5
  • Negative Genetic: 6
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 7
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 58
  • Synthetic Rescue: 34

Resources
Expression Summary
histogram
Resources
Length (a.a.) 640
Molecular Weight (Da) 72,922
Isoelectric Point (pI) 6.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:184220 to 182298 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -87 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1923 184220..182298 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003663
SUMMARY PARAGRAPH for SPT10

SPT10 is one member of a large class of SPT genes, which were named for their ability to suppress the phenotypes resulting from Ty insertions that disrupted transcription of nearby genes (8). Genes with this phenotype include the TATA binding protein, some histones, regulators of histone gene expression, and members of the SAGA histone acetyltransferase complex (8). Spt10p is thought to be involved in chromatin maintenance and transcriptional regulation, and mutations in Spt10p are suppressed by the transcriptional regulator Ccr4p (9, 10). Spt10p is required for transcription of some but not all of the histone genes (11). It is not an essential gene, but deletion of SPT10 causes very slow growth and results in increased expression from several otherwise repressed promoters (3, 1, 12). One domain of Spt10p has similarity to a Zn-finger motif, and this domain is associated with Spt10p's transcriptional regulation (but is not involved in the slow growth phenotype of the mutant) (1).

Last updated: 1999-12-31 Contact SGD

References cited on this page View Complete Literature Guide for SPT10
1) Natsoulis G, et al.  (1994) The SPT10 and SPT21 genes of Saccharomyces cerevisiae. Genetics 136(1):93-105
2) Denis CL  (1984) Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Genetics 108(4):833-44
3) Yamashita I  (1993) Isolation and characterization of the SUD1 gene, which encodes a global repressor of core promoter activity in Saccharomyces cerevisiae. Mol Gen Genet 241(5-6):616-26
4) Shen CH, et al.  (2002) Targeted histone acetylation at the yeast CUP1 promoter requires the transcriptional activator, the TATA boxes, and the putative histone acetylase encoded by SPT10. Mol Cell Biol 22(18):6406-16
5) Eriksson PR, et al.  (2005) Global regulation by the yeast Spt10 protein is mediated through chromatin structure and the histone upstream activating sequence elements. Mol Cell Biol 25(20):9127-37
6) Chang JS and Winston F  (2011) Spt10 and Spt21 Are Required for Transcriptional Silencing in Saccharomyces cerevisiae. Eukaryot Cell 10(1):118-29
7) Fassler JS and Winston F  (1988) Isolation and analysis of a novel class of suppressor of Ty insertion mutations in Saccharomyces cerevisiae. Genetics 118(2):203-12
8) Winston F and Sudarsanam P  (1998) The SAGA of Spt proteins and transcriptional analysis in yeast: past, present, and future. Cold Spring Harb Symp Quant Biol 63:553-61
9) Draper MP, et al.  (1994) CCR4 is a glucose-regulated transcription factor whose leucine-rich repeat binds several proteins important for placing CCR4 in its proper promoter context. Mol Cell Biol 14(7):4522-31
10) Denis CL, et al.  (1994) The yeast CCR4 protein is neither regulated by nor associated with the SPT6 and SPT10 proteins and forms a functionally distinct complex from that of the SNF/SWI transcription factors. Genetics 138(4):1005-13
11) Dollard C, et al.  (1994) SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. Mol Cell Biol 14(8):5223-8
12) Natsoulis G, et al.  (1991) The products of the SPT10 and SPT21 genes of Saccharomyces cerevisiae increase the amplitude of transcriptional regulation at a large number of unlinked loci. New Biol 3(12):1249-59