SPP382/YLR424W Summary Help

Standard Name SPP382 1, 2
Systematic Name YLR424W
Alias CCF8 3 , NTR1 4
Feature Type ORF, Verified
Description Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation (2, 3, 4)
Name Description Suppressor of PrP38 #2 2
Chromosomal Location
ChrXII:973395 to 975521 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPP382 GO evidence and references
  View Computational GO annotations for SPP382
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
null
reduction of function
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
171 total interaction(s) for 86 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 79
  • Affinity Capture-Western: 13
  • Biochemical Activity: 1
  • Co-fractionation: 2
  • PCA: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 36

Genetic Interactions
  • Dosage Lethality: 9
  • Dosage Rescue: 3
  • Negative Genetic: 17
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 708
Molecular Weight (Da) 83,048
Isoelectric Point (pI) 5.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:973395 to 975521 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2127 973395..975521 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004416
References cited on this page View Complete Literature Guide for SPP382
1) Lebaron S, et al.  (2005) The splicing ATPase prp43p is a component of multiple preribosomal particles. Mol Cell Biol 25(21):9269-82
2) Pandit S, et al.  (2006) Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A 103(37):13700-5
3) Wang Q, et al.  (2003) The Clf1p splicing factor promotes spliceosome assembly through N-terminal tetratricopeptide repeat contacts. J Biol Chem 278(10):7875-83
4) Tsai RT, et al.  (2005) Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. Genes Dev 19(24):2991-3003