SPP1/YPL138C Summary Help

Standard Name SPP1 1
Systematic Name YPL138C
Alias CPS40 2 , SAF41 3
Feature Type ORF, Verified
Description Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Set1c, Phd finger Protein
Chromosomal Location
ChrXVI:292427 to 291366 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SPP1 GO evidence and references
  View Computational GO annotations for SPP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 59 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
188 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-Luminescence: 2
  • Affinity Capture-MS: 52
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 10
  • Biochemical Activity: 4
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 4
  • Protein-peptide: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 18

Genetic Interactions
  • Negative Genetic: 35
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Positive Genetic: 5
  • Synthetic Growth Defect: 20
  • Synthetic Lethality: 16
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 353
Molecular Weight (Da) 41,468
Isoelectric Point (pI) 6.73
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:292427 to 291366 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1062 292427..291366 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006059
References cited on this page View Complete Literature Guide for SPP1
1) Roguev A, et al.  (2001) The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J 20(24):7137-48
2) Miller T, et al.  (2001) COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Proc Natl Acad Sci U S A 98(23):12902-7
3) Nagy PL, et al.  (2002) A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc Natl Acad Sci U S A 99(1):90-4
4) Krogan NJ, et al.  (2002) COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J Biol Chem 277(13):10753-5
5) Dover J, et al.  (2002) Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6. J Biol Chem 277(32):28368-71
6) Cheng H, et al.  (2004) The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3. Mol Cell Biol 24(7):2932-43
7) Kan J, et al.  (2008) Origin Recognition Complex (ORC) Mediates Histone 3 Lysine 4 Methylation through Cooperation with Spp1 in Saccharomyces cerevisiae. J Biol Chem 283(49):33803-7
8) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30