Standard Name	SPO14 1
Systematic Name	YKR031C
Alias	PLD1
Feature Type	ORF, Verified
Description	Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with "foot domain" of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions (2, 3, 4, 5, 6)
Name Description	SPOrulation 7

Chromosomal Location	ChrXI:506395 to 501344 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 18 cM

	View Computational GO annotations for SPO14
Molecular Function
Manually curated	phosphatidylinositol-3-phosphate binding (IDA) phospholipase D activity (IDA)
Biological Process
Manually curated	ascospore-type prospore assembly (IMP) cell morphogenesis involved in conjugation with cellular fusion (IGI, IMP) exocytosis (IGI) phospholipid metabolic process (IDA, IMP)
Cellular Component
Manually curated	endosome (IDA) nucleus (IDA) prospore membrane (IDA)

Pathways	phospholipids degradation

Classical genetics
null	N-palmitoylethanolamine accumulation: decreased meiosis: decreased resistance to 1-O-hexadecyl-2-acetyl-sn-glycerophosphocholine: decreased sporulation: absent
Large-scale survey
null	competitive fitness: normal hyperosmotic stress resistance: decreased resistance to edelfosine: decreased resistance to arsenite(3-): decreased sporulation: absent sporulation: abnormal sporulation efficiency: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	81 total interaction(s) for 65 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Co-localization: 2 Two-hybrid: 16
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 16 Phenotypic Suppression: 8 Positive Genetic: 3 Synthetic Growth Defect: 14 Synthetic Lethality: 8 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:506395 to 501344 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
	Genetic position: 18 cM
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..5052 506395..501344 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001739