SPN1/YPR133C Summary Help

Standard Name SPN1 1
Systematic Name YPR133C
Alias IWS1 2
Feature Type ORF, Verified
Description Protein involved in RNA polymerase II transcription; is constitutively recruited to the CYC1 promoter and is required for recruitment of chromatin remodeling factors for the expression of CYC1 gene; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype (1, 2, 3, 4, 5)
Name Description Suppresses Postrecruitment functions gene Number 1 1
Chromosomal Location
ChrXVI:797210 to 795978 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SPN1 GO evidence and references
  View Computational GO annotations for SPN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 122 genes
Regulators 1 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
unspecified
Resources
112 total interaction(s) for 88 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 5
  • Co-crystal Structure: 1
  • PCA: 5
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 57
  • Phenotypic Suppression: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 4
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 410
Molecular Weight (Da) 46,082
Isoelectric Point (pI) 7.81
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:797210 to 795978 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1233 797210..795978 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006337
References cited on this page View Complete Literature Guide for SPN1
1) Fischbeck JA, et al.  (2002) SPN1, a conserved gene identified by suppression of a postrecruitment-defective yeast TATA-binding protein mutant. Genetics 162(4):1605-16
2) Krogan NJ, et al.  (2002) RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol Cell Biol 22(20):6979-92
3) Lindstrom DL, et al.  (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78
4) Zhang L, et al.  (2008) Spn1 regulates the recruitment of Spt6 and the Swi/Snf complex during transcriptional activation by RNA polymerase II. Mol Cell Biol 28(4):1393-403
5) Pujari V, et al.  (2010) The Transcription Factor Spn1 Regulates Gene Expression via a Highly Conserved Novel Structural Motif. J Mol Biol 404(1):1-15