SPL2/YHR136C Summary Help

Standard Name SPL2 1
Systematic Name YHR136C
Feature Type ORF, Verified
Description Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm (1, 2, 3, 4, 5, 6)
Name Description Suppressor of PLc1 deletion 1
Chromosomal Location
ChrVIII:375100 to 374654 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SPL2 GO evidence and references
  View Computational GO annotations for SPL2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 23 genes
Resources
Classical genetics
null
Large-scale survey
null
repressible
Resources
15 total interaction(s) for 10 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • PCA: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 4
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 148
Molecular Weight (Da) 17,036
Isoelectric Point (pI) 6.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:375100 to 374654 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..447 375100..374654 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001178
References cited on this page View Complete Literature Guide for SPL2
1) Flick JS and Thorner J  (1998) An essential function of a phosphoinositide-specific phospholipase C is relieved by inhibition of a cyclin-dependent protein kinase in the yeast Saccharomyces cerevisiae. Genetics 148(1):33-47
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Ferreira TC, et al.  (2007) The yeast genome may harbor hypoxia response elements (HRE). Comp Biochem Physiol C Toxicol Pharmacol 146(1-2):255-63
4) Wykoff DD, et al.  (2007) Positive feedback regulates switching of phosphate transporters in S. cerevisiae. Mol Cell 27(6):1005-13
5) Shivaswamy S and Iyer VR  (2008) Stress-dependent dynamics of global chromatin remodeling in yeast: dual role for SWI/SNF in the heat shock stress response. Mol Cell Biol 28(7):2221-34
6) Ghillebert R, et al.  (2011) Differential roles for the low-affinity phosphate transporters Pho87 and Pho90 in Saccharomyces cerevisiae. Biochem J 434(2):243-51