SPE1/YKL184W Summary Help

Standard Name SPE1 1
Systematic Name YKL184W
Alias ORD1 , SPE10 2
Feature Type ORF, Verified
Description Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation (3, 4, 5, 6)
Name Description SPErmidine auxotroph 1
Chromosomal Location
ChrXI:96757 to 98157 | ORF Map | GBrowse
Gbrowse
Genetic position: -130.3 cM
Gene Ontology Annotations All SPE1 GO evidence and references
  View Computational GO annotations for SPE1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 4 genes
Resources
Pathways
Classical genetics
null
unspecified
Large-scale survey
null
repressible
unspecified
Resources
287 total interaction(s) for 205 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 178
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 2
  • Positive Genetic: 79
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 1
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 466
Molecular Weight (Da) 52,285
Isoelectric Point (pI) 5.31
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:96757 to 98157 | ORF Map | GBrowse
SGD ORF map
Genetic position: -130.3 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1401 96757..98157 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001667
References cited on this page View Complete Literature Guide for SPE1
1) Whitney PA and Morris DR  (1978) Polyamine auxotrophs of Saccharomyces cerevisiae. J Bacteriol 134(1):214-20
2) Xie QW, et al.  (1990) Ornithine decarboxylase in Saccharomyces cerevisiae: chromosomal assignment and genetic mapping of the SPE1 gene. Yeast 6(6):455-60
3) Toth C and Coffino P  (1999) Regulated degradation of yeast ornithine decarboxylase. J Biol Chem 274(36):25921-6
4) Fonzi WA and Sypherd PS  (1987) The gene and the primary structure of ornithine decarboxylase from Saccharomyces cerevisiae. J Biol Chem 262(21):10127-33
5) Fonzi WA  (1989) Biochemical and genetic characterization of the structure of yeast ornithine decarboxylase. Biochem Biophys Res Commun 162(3):1409-16
6) Eisenberg T, et al.  (2009) Induction of autophagy by spermidine promotes longevity. Nat Cell Biol 11(11):1305-14