SPC2/YML055W Summary

Standard Name	SPC2
Systematic Name	YML055W
Alias	SPY1
Feature Type	ORF, Verified
Description	Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 (1, 2, 3, 4 and see Summary Paragraph)
Name Description	Signal Peptidase Complex 1

Chromosomal Location	ChrXIII:164790 to 165326 \| ORF Map \| GBrowse

Gene Ontology Annotations All SPC2 GO evidence and references

	View Computational GO annotations for SPC2
Molecular Function
Manually curated	contributes_to peptidase activity (IMP)
Biological Process
Manually curated	protein targeting to ER (IGI, IMP, IPI) signal peptide processing (IGI, IMP, IPI, ISS)
Cellular Component
Manually curated	signal peptidase complex (IDA)

Mutant phenotypes All SPC2 Phenotype evidence and references

Classical genetics
null	resistance to mercaptoethanol: normal resistance to L-1,4-dithiothreitol: decreased resistance to tunicamycin: decreased
overexpression	nuclear morphology: abnormal
Large-scale survey
null	auxotrophy competitive fitness: decreased resistance to caffeine: decreased resistance to camptothecin: decreased resistance to bleomycin: decreased resistance to quinine: decreased resistance to sirolimus: increased resistance to cycloheximide: increased resistance to fenpropimorph: decreased resistance to methyl methanesulfonate: decreased resistance to mycophenolic acid: decreased stress resistance: decreased vacuolar morphology: abnormal viable
overexpression	cell cycle progression: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SPC2 Interaction evidence and references

	149 total interaction(s) for 75 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 PCA: 7 Two-hybrid: 5
Genetic Interactions	Negative Genetic: 74 Phenotypic Enhancement: 10 Phenotypic Suppression: 33 Positive Genetic: 6 Synthetic Growth Defect: 3 Synthetic Lethality: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SPC2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIII:164790 to 165326 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..537	164790..165326	1996-07-31	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004519

SUMMARY PARAGRAPH for SPC2

SPC2 encodes a subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER) (1). Signal peptide cleavage occurs concomitantly with translocation through the translocon pore into the ER. In yeast, translocation can occur cotranslationally or posttranslationally, whereas in mammals translocation is always cotranslational. The process of protein translocation into the ER is reviewed in references 5 and 6.

The yeast SPC comprises four proteins, Spc1p, Spc2p, Spc3p, and Sec11p (7, 4). Spc2p is homologous to the mammalian signal peptidase subunit SPC25 (1). Spc2p is not essential for viability or for signal peptidase activity (1, 4), but deletion of SPC2 reduces signal peptidase activity in vitro (4). The spc2 null mutant also shows reduced levels of the other SPC subunits (4), and is synthetically lethal with a temperature-sensitive mutation in SEC11 (1).

Spc2p can be coimmunoprecipitated with Sbh1p and Sbh2p, which are beta subunits of two different "Sec61" complexes involved in ER membrane translocation (4, 6). The genetic and physical interactions observed suggest that Spc2p may facilitate interactions between different components of the translocation machinery (4).

Last updated: 2000-11-28

References cited on this page View Complete Literature Guide for SPC2

1)	Mullins C, et al. (1996) Structurally related Spc1p and Spc2p of yeast signal peptidase complex are functionally distinct. J Biol Chem 271(46):29094-9
2)	Fang H, et al. (1997) In addition to SEC11, a newly identified gene, SPC3, is essential for signal peptidase activity in the yeast endoplasmic reticulum. J Biol Chem 272(20):13152-8
3)	Meyer HA and Hartmann E (1997) The yeast SPC22/23 homolog Spc3p is essential for signal peptidase activity. J Biol Chem 272(20):13159-64
4)	Antonin W, et al. (2000) Interactions between Spc2p and other components of the endoplasmic reticulum translocation sites of the yeast Saccharomyces cerevisiae. J Biol Chem 275(44):34068-72
5)	Rapoport TA, et al. (1996) Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes. Annu Rev Biochem 65:271-303
6)	Johnson AE and van Waes MA (1999) The translocon: a dynamic gateway at the ER membrane. Annu Rev Cell Dev Biol 15:799-842
7)	YaDeau JT, et al. (1991) Yeast signal peptidase contains a glycoprotein and the Sec11 gene product. Proc Natl Acad Sci U S A 88(2):517-21