SPC19/YDR201W Summary Help

Standard Name SPC19
Systematic Name YDR201W
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body (1, 2, 3, 4, 5)
Name Description Spindle Pole Component
Chromosomal Location
ChrIV:856317 to 856814 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPC19 GO evidence and references
  View Computational GO annotations for SPC19
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
repressible
Resources
108 total interaction(s) for 49 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 25
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Co-fractionation: 2
  • Co-purification: 7
  • PCA: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 60

Genetic Interactions
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 165
Molecular Weight (Da) 18,909
Isoelectric Point (pI) 4.59
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:856317 to 856814 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..498 856317..856814 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002609
References cited on this page View Complete Literature Guide for SPC19
1) Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77
2) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
3) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
4) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
5) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9