SPC110/YDR356W Summary Help

Standard Name SPC110 1
Systematic Name YDR356W
Alias XCM1 2 , NUF1 3
Feature Type ORF, Verified
Description Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner (2, 3, 4, 5, 6)
Name Description Spindle Pole Component 1
Chromosomal Location
ChrIV:1186107 to 1188941 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPC110 GO evidence and references
  View Computational GO annotations for SPC110
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
reduction of function
repressible
Resources
206 total interaction(s) for 118 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 16
  • Biochemical Activity: 7
  • Co-localization: 1
  • Co-purification: 40
  • FRET: 10
  • Reconstituted Complex: 9
  • Two-hybrid: 20

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 4
  • Dosage Rescue: 15
  • Negative Genetic: 2
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 3
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 50
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 944
Molecular Weight (Da) 111,781
Isoelectric Point (pI) 7.08
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1186107 to 1188941 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2835 1186107..1188941 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002764
References cited on this page View Complete Literature Guide for SPC110
1) Kilmartin JV, et al.  (1993) A spacer protein in the Saccharomyces cerevisiae spindle poly body whose transcript is cell cycle-regulated. J Cell Biol 123(5):1175-84
2) Geiser JR, et al.  (1993) The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae. Mol Cell Biol 13(12):7913-24
3) Mirzayan C, et al.  (1992) The NUF1 gene encodes an essential coiled-coil related protein that is a potential component of the yeast nucleoskeleton. J Cell Biol 116(6):1319-32
4) Flory MR, et al.  (2000) Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin. Proc Natl Acad Sci U S A 97(11):5919-23
5) Knop M and Schiebel E  (1997) Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p. EMBO J 16(23):6985-95
6) Friedman DB, et al.  (2001) Yeast Mps1p phosphorylates the spindle pole component Spc110p in the N-terminal domain. J Biol Chem 276(21):17958-67