SPC105/YGL093W Summary Help

Standard Name SPC105 1
Systematic Name YGL093W
Feature Type ORF, Verified
Description Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Kre28p; modified by sumoylation (2, 3, 4)
Name Description Spindle Pole Component 1
Chromosomal Location
ChrVII:334886 to 337639 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPC105 GO evidence and references
  View Computational GO annotations for SPC105
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
null
Large-scale survey
conditional
null
Resources
112 total interaction(s) for 73 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-Western: 12
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 9
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 57

Genetic Interactions
  • Dosage Rescue: 2
  • Positive Genetic: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 917
Molecular Weight (Da) 104,825
Isoelectric Point (pI) 4.97
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:334886 to 337639 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2754 334886..337639 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003061
References cited on this page View Complete Literature Guide for SPC105
1) Wigge PA, et al.  (1998) Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol 141(4):967-77
2) Nekrasov VS, et al.  (2003) Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell 14(12):4931-46
3) Pagliuca C, et al.  (2009) Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint. PLoS One 4(10):e7640
4) Yong-Gonzales V, et al.  (2012) The Smc5-Smc6 Complex Regulates Recombination at Centromeric Regions and Affects Kinetochore Protein Sumoylation during Normal Growth. PLoS One 7(12):e51540