SPC1/YJR010C-A Summary

Standard Name	SPC1
Systematic Name	YJR010C-A
Feature Type	ORF, Verified
Description	Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress (1, 2, 3 and see Summary Paragraph)
Name Description	Signal Peptidase Complex 1

Chromosomal Location	ChrX:458361 to 458077 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All SPC1 GO evidence and references

	View Computational GO annotations for SPC1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	protein targeting to ER (IGI, IPI) signal peptide processing (IGI, IMP, IPI, ISS)
Cellular Component
Manually curated	signal peptidase complex (IDA)

Mutant phenotypes All SPC1 Phenotype evidence and references

Large-scale survey
null	competitive fitness: increased endocytosis: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SPC1 Interaction evidence and references

	113 total interaction(s) for 87 unique genes/features.
Physical Interactions	Affinity Capture-MS: 32 Affinity Capture-Western: 2 Co-purification: 1 PCA: 46 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 15 Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Positive Genetic: 3 Synthetic Growth Defect: 3 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SPC1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:458361 to 458077 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..285	458361..458077	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003770

SUMMARY PARAGRAPH for SPC1

SPC1 encodes a subunit of the signal peptidase complex (SPC), which cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER) (1). Signal peptide cleavage occurs concomitantly with translocation through the translocon pore into the ER. In yeast, translocation can occur cotranslationally or posttranslationally, whereas in mammals translocation is always cotranslational. The process of protein translocation into the ER is reviewed in references 4 and 5.

The yeast SPC comprises four proteins, Spc1p, Spc2p, Spc3p, and Sec11p (6, 2). Spc1p is homologous to the mammalian signal peptidase subunit SPC12 (1). Spc1p is not essential for viability or for signal peptidase activity in vivo or in vitro (1, 2), and cells lacking both Spc1p and Spc2p are also viable (7). Deletion of SPC1 is synthetically lethal with a temperature-sensitive mutation in SEC11, which encodes the catalytic SPC subunit (7). Overexpression of SPC1 suppresses the sec11 temperature sensitive phenotype (1).

Last updated: 2000-11-27

References cited on this page View Complete Literature Guide for SPC1

1)	Fang H, et al. (1996) The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae. J Biol Chem 271(28):16460-5
2)	Antonin W, et al. (2000) Interactions between Spc2p and other components of the endoplasmic reticulum translocation sites of the yeast Saccharomyces cerevisiae. J Biol Chem 275(44):34068-72
3)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4)	Rapoport TA, et al. (1996) Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes. Annu Rev Biochem 65:271-303
5)	Johnson AE and van Waes MA (1999) The translocon: a dynamic gateway at the ER membrane. Annu Rev Cell Dev Biol 15:799-842
6)	YaDeau JT, et al. (1991) Yeast signal peptidase contains a glycoprotein and the Sec11 gene product. Proc Natl Acad Sci U S A 88(2):517-21
7)	Mullins C, et al. (1996) Structurally related Spc1p and Spc2p of yeast signal peptidase complex are functionally distinct. J Biol Chem 271(46):29094-9