SPB1/YCL054W Summary Help

Standard Name SPB1 1
Systematic Name YCL054W
Feature Type ORF, Verified
Description AdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants (2, 3, 4)
Name Description Suppressor of PaB1 mutant 1
Chromosomal Location
ChrIII:31449 to 33974 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SPB1 GO evidence and references
  View Computational GO annotations for SPB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Resources
Classical genetics
conditional
null
reduction of function
unspecified
Large-scale survey
null
reduction of function
Resources
58 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 43
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions
  • Positive Genetic: 3
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 841
Molecular Weight (Da) 96,484
Isoelectric Point (pI) 8.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIII:31449 to 33974 | ORF Map | GBrowse
This feature contains embedded feature(s): YCL053C
SGD ORF map
Last Update Coordinates: 2000-09-13 | Sequence: 1999-07-17
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2526 31449..33974 2000-09-13 1999-07-17
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000559
References cited on this page View Complete Literature Guide for SPB1
1) Sachs AB and Davis RW  (1989) The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation. Cell 58(5):857-67
2) Kressler D, et al.  (1999) Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae. Nucleic Acids Res 27(23):4598-608
3) Bonnerot C, et al.  (2003) Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast. Mol Cell 12(5):1309-15
4) Lapeyre B and Purushothaman SK  (2004) Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage. Mol Cell 16(4):663-9