Standard Name	SPB1
Systematic Name	YCL054W
Feature Type	ORF, Verified
Description	AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants (1, 2, 3)
Name Description	Suppressor of PaB1 mutant 4

Chromosomal Location	ChrIII:31449 to 33974 | ORF Map | GBrowse

	View Computational GO annotations for SPB1
Molecular Function
Manually curated	rRNA (guanine) methyltransferase activity (IMP) rRNA (uridine-2'-O-)-methyltransferase activity (IGI)
Biological Process
Manually curated	maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) (IMP) rRNA methylation (IDA, IGI, IMP)
Cellular Component
Manually curated	nucleolus (IDA) nucleus (IDA) preribosome, large subunit precursor (IDA)

Classical genetics
null	inviable sporulation: absent
reduction of function	growth in exponential phase: decreased rate vegetative growth: decreased
unspecified	cold sensitivity: increased resistance to cycloheximide: decreased viable
Large-scale survey
null	haploinsufficient inviable
reduction of function	competitive fitness: decreased resistance to methyl methanesulfonate: decreased resistance to hydroxyurea: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	53 total interaction(s) for 39 unique genes/features.
Physical Interactions	Affinity Capture-MS: 39 Affinity Capture-RNA: 2 Affinity Capture-Western: 3 Biochemical Activity: 3 Two-hybrid: 1
Genetic Interactions	Positive Genetic: 3 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIII:31449 to 33974 | ORF Map | GBrowse
	This feature contains embedded feature(s): YCL053C
Last Update	Coordinates: 2000-09-13 | Sequence: 1999-07-17
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2526 31449..33974 2000-09-13 1999-07-17
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000559