SPA2/YLL021W Summary Help

Standard Name SPA2 1
Systematic Name YLL021W
Alias FUS6 2 , PEA1 3 , PPF1 4
Feature Type ORF, Verified
Description Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication (5, 6, 7, 8, 9)
Name Description Spindle Pole Antigen 1
Chromosomal Location
ChrXII:100947 to 105347 | ORF Map | GBrowse
Genetic position: -28 cM
Gene Ontology Annotations All SPA2 GO evidence and references
  View Computational GO annotations for SPA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Classical genetics
reduction of function
Large-scale survey
165 total interaction(s) for 102 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 5
  • Biochemical Activity: 1
  • Co-localization: 3
  • PCA: 5
  • Reconstituted Complex: 3
  • Two-hybrid: 23

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 14
  • Negative Genetic: 35
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 5
  • Positive Genetic: 1
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 23
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 1,466
Molecular Weight (Da) 163,141
Isoelectric Point (pI) 4.92
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:100947 to 105347 | ORF Map | GBrowse
Genetic position: -28 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4401 100947..105347 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003944
References cited on this page View Complete Literature Guide for SPA2
1) Snyder M  (1989) The SPA2 protein of yeast localizes to sites of cell growth. J Cell Biol 108(4):1419-29
2) Gammie AE, et al.  (1998) Distinct morphological phenotypes of cell fusion mutants. Mol Biol Cell 9(6):1395-410
3) Chenevert J, et al.  (1994) Identification of genes required for normal pheromone-induced cell polarization in Saccharomyces cerevisiae. Genetics 136(4):1287-96
4) Yorihuzi T and Ohsumi Y  (1994) Saccharomyces cerevisiae MATa mutant cells defective in pointed projection formation in response to alpha-factor at high concentrations. Yeast 10(5):579-94
5) Sheu YJ, et al.  (1998) Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis. Mol Cell Biol 18(7):4053-69
6) van Drogen F and Peter M  (2002) Spa2p functions as a scaffold-like protein to recruit the Mpk1p MAP kinase module to sites of polarized growth. Curr Biol 12(19):1698-703
7) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
8) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
9) Carvalho-Netto OV, et al.  (2013) A simple and effective set of PCR-based molecular markers for the monitoring of the Saccharomyces cerevisiae cell population during bioethanol fermentation. J Biotechnol 168(4):701-9