SOL4/YGR248W Summary Help

Standard Name SOL4 1
Systematic Name YGR248W
Feature Type ORF, Verified
Description 6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication (1, 2, 3 and see Summary Paragraph)
Name Description Suppressor Of Los1-1 1
Chromosomal Location
ChrVII:985972 to 986739 | ORF Map | GBrowse
Gene Ontology Annotations All SOL4 GO evidence and references
  View Computational GO annotations for SOL4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 13 genes
Classical genetics
Large-scale survey
54 total interaction(s) for 41 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 4
  • Co-purification: 1

Genetic Interactions
  • Negative Genetic: 39
  • Positive Genetic: 7
  • Synthetic Growth Defect: 1

Expression Summary
Length (a.a.) 255
Molecular Weight (Da) 28,447
Isoelectric Point (pI) 5.11
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:985972 to 986739 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..768 985972..986739 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003480

The Saccharomyces cerevisiae SOL protein family includes Sol1p, Sol2p, Sol3p, and Sol4p, and is unusual in that its individual members are biochemically distinct and spatially dispersed. Sol1p and Sol2p appear to function in tRNA nuclear export, as determined by mutant studies (1). Sol3p and Sol4p both exhibit 6-phosphogluconolactonase activity (EC and function in the pentose phosphate pathway (1, 4). Although different analyses have produced slightly different results, Sol1p appears to localize mostly within the nucleus whereas Sol2p, Sol3p and Sol4p localize predominantly in the cytosol (1, 5). SOL1 is an efficient multi-copy suppressor of the loss of nonsense suppression defect displayed by los1 mutants, as is SOL2, although to a lesser extent. In contrast, Sol3p is only a very weak multi-copy suppressor of los1 mutations and Sol4p does not function in this capacity at all (1).

Null mutants in any or all of the four SOL genes are viable. Nulls in sol1 or sol2 display normal levels of 6-phosphogluconolactonase activity, but possess elevated levels of nuclear tRNA, indicating a defect in tRNA export from the nucleus (1). Null mutants in either sol3 or sol4 display reduced levels of 6-phosphogluconolactonase activity but possess normal levels of nuclear tRNA (1). The viability of quadruple null mutants in sol1-4 indicates that this gene family is not essential, but these mutants display no detectable 6-phosphogluconolactonase activity and do possess elevated levels of nuclear tRNA (1).

Sol1p, Sol2p, Sol3p and Sol4p have similarity to each other, and to Candida albicans Sol1p, Schizosaccharomyces pombe Sol1p, human PGLS which is associated with 6-phosphogluconolactonase deficiency, and human H6PD which is associated with cortisone reductase deficiency. Sol1p, Sol2p, Sol3p and Sol4p are also similar to the 6-phosphogluconolactonases in bacteria (Pseudomonas aeruginosa) and eukaryotes (Drosophila melanogaster, Arabidopsis thaliana, and Trypanosoma brucei), to the glucose-6-phosphate dehydrogenase enzymes from bacteria (Mycobacterium leprae) and eukaryotes (Plasmodium falciparum and rabbit liver microsomes), and have regions of similarity to proteins of the Nag family, including human GNPI and Escherichia coli NagB (1, 6, 7, 8).

Last updated: 2008-02-18 Contact SGD

References cited on this page View Complete Literature Guide for SOL4
1) Stanford DR, et al.  (2004) Division of labor among the yeast Sol proteins implicated in tRNA nuclear export and carbohydrate metabolism. Genetics 168(1):117-27
2) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
3) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4) Blank LM, et al.  (2005) Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast. Genome Biol 6(6):R49
5) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
6) Shen WC, et al.  (1996) Los1p, involved in yeast pre-tRNA splicing, positively regulates members of the SOL gene family. Genetics 143(2):699-712
7) Collard F, et al.  (1999) Identification of the cDNA encoding human 6-phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway(1). FEBS Lett 459(2):223-6
8) Duffieux F, et al.  (2000) Molecular characterization of the first two enzymes of the pentose-phosphate pathway of Trypanosoma brucei. Glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase. J Biol Chem 275(36):27559-65