Standard Name	SNU114 1
Systematic Name	YKL173W
Alias	GIN10
Feature Type	ORF, Verified
Description	GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 (1, 2, 3, 4)
Name Description	Small NUclear ribonucleoprotein associated 1

Chromosomal Location	ChrXI:122517 to 125543 | ORF Map | GBrowse

	View Computational GO annotations for SNU114
Molecular Function
Manually curated	GTP binding (ISS) GTPase activity (IMP, ISS) U5 snRNA binding (IDA)
Biological Process
Manually curated	assembly of spliceosomal tri-snRNP (IMP) generation of catalytic spliceosome for first transesterification step (IGI) mRNA splicing, via spliceosome (IMP) spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) (IMP)
Cellular Component
Manually curated	U4/U6 x U5 tri-snRNP complex (IDA) U5 snRNP (IDA)

Classical genetics
conditional	cold sensitivity: increased heat sensitivity: increased
null	inviable
reduction of function	vegetative growth: decreased rate
repressible	unspliced U3 pre-mRNA accumulation: increased
Large-scale survey
conditional	colony sectoring: increased resistance to benomyl: decreased resistance to methyl methanesulfonate: decreased
null	inviable starvation resistance: decreased
reduction of function	chromosome/plasmid maintenance: abnormal colony sectoring: increased competitive fitness: decreased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	253 total interaction(s) for 131 unique genes/features.
Physical Interactions	Affinity Capture-MS: 166 Affinity Capture-Western: 9 Co-fractionation: 2 Co-localization: 1 Co-purification: 3 PCA: 3 Reconstituted Complex: 1 Two-hybrid: 3
Genetic Interactions	Negative Genetic: 42 Positive Genetic: 5 Synthetic Growth Defect: 2 Synthetic Lethality: 16
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Mass Spec | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:122517 to 125543 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3027 122517..125543 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001656