SNQ2/YDR011W Summary Help

Standard Name SNQ2 1
Systematic Name YDR011W
Feature Type ORF, Verified
Description Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species (2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description Sensitivity to 4-NitroQuinoline-N-oxide 1
Chromosomal Location
ChrIV:465919 to 470424 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SNQ2 GO evidence and references
  View Computational GO annotations for SNQ2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All SNQ2 Phenotype evidence and references
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
interactions All SNQ2 Interaction evidence and references
47 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 1
  • Co-fractionation: 3
  • PCA: 19
  • Protein-RNA: 1
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 1
  • Phenotypic Enhancement: 2
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All SNQ2 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:465919 to 470424 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..4506 465919..470424 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002418
SUMMARY PARAGRAPH for SNQ2

SNQ2 encodes a plasma membrane multidrug transporter that is involved in the pleiotropic drug response (7, 1). Snq2p efflux pump activity is ATP-dependent (7) and mediates resistance to many xenobiotic compounds including mutagens, fungicides, steroids, and anticancer drugs (reviewed in 8 and 9). Null mutations of snq2 are not lethal but do confer a drug- and salt-hypersensitive phenotype (reviewed in 8 and 9). In addition to drug response, Snq2p is also involved in cation resistance (10) and in quorum sensing for yeast populations growing in liquid culture (11).

SNQ2 basal levels of expression are positively regulated by Pdr1p, Pdr3p, and Stb5p, through the binding of these transcription factors to pleiotropic drug response elements (PDREs) present in the SNQ2 promoter (7, 12, 13, and 14). SNQ2 expression is also drug-induced by Yrr1p (15, 16) and heat-shock-induced by the AP-1 transcription factors Yap1p and Cad1p (17).

Snq2p is a member of the ATP-binding cassette (ABC) family of proteins, a large group that are conserved from bacteria to humans (1, 2). Overexpression of the human ABC transporter ABCB1/MDR1 (OMIM) is a factor in tumor resistance to drug therapy, and deficient ABC transporter function has been implicated in other human diseases as well (reviewed in 2). S. cerevisiae ABC proteins are often used as a model to study the clinical problem of drug resistance in infectious disease and cancer as well as in pharmaceutical screens for novel drugs (18 and reviewed in 19).

Last updated: 2011-01-13

References cited on this page View Complete Literature Guide for SNQ2
1) Servos J, et al.  (1993) Gene SNQ2 of Saccharomyces cerevisiae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases. Mol Gen Genet 236(2-3):214-8
2) Decottignies A and Goffeau A  (1997) Complete inventory of the yeast ABC proteins. Nat Genet 15(2):137-45
3) Rogers B, et al.  (2001) The pleitropic drug ABC transporters from Saccharomyces cerevisiae. J Mol Microbiol Biotechnol 3(2):207-14
4) Ververidis P, et al.  (2001) A novel putative reductase (Cpd1p) and the multidrug exporter Snq2p are involved in resistance to cercosporin and other singlet oxygen-generating photosensitizers in Saccharomyces cerevisiae. Curr Genet 39(3):127-36
5) Navarre C, et al.  (2002) Subproteomics: identification of plasma membrane proteins from the yeast Saccharomyces cerevisiae. Proteomics 2(12):1706-14
6) Anderson JB, et al.  (2003) Mode of selection and experimental evolution of antifungal drug resistance in Saccharomyces cerevisiae. Genetics 163(4):1287-98
7) Decottignies A, et al.  (1995) Identification and characterization of SNQ2, a new multidrug ATP binding cassette transporter of the yeast plasma membrane. J Biol Chem 270(30):18150-7
8) Ernst R, et al.  (2005) Yeast ATP-binding cassette transporters: cellular cleaning pumps. Methods Enzymol 400:460-84
9) Bauer BE, et al.  (1999) Inventory and function of yeast ABC proteins: about sex, stress, pleiotropic drug and heavy metal resistance. Biochim Biophys Acta 1461(2):217-36
10) Miyahara K, et al.  (1996) The involvement of the Saccharomyces cerevisiae multidrug resistance transporters Pdr5p and Snq2p in cation resistance. FEBS Lett 399(3):317-20
11) Hlavacek O, et al.  (2009) Putative role for ABC multidrug exporters in yeast quorum sensing. FEBS Lett 583(7):1107-13
12) Mahe Y, et al.  (1996) The ATP-binding cassette multidrug transporter Snq2 of Saccharomyces cerevisiae: a novel target for the transcription factors Pdr1 and Pdr3. Mol Microbiol 20(1):109-17
13) Akache B and Turcotte B  (2002) New regulators of drug sensitivity in the family of yeast zinc cluster proteins. J Biol Chem 277(24):21254-60
14) Cui Z, et al.  (1999) Functional analysis of the promoter of the yeast SNQ2 gene encoding a multidrug resistance transporter that confers the resistance to 4-nitroquinoline N-oxide. Biosci Biotechnol Biochem 63(1):162-7
15) Cui Z, et al.  (1998) Yeast gene YRR1, which is required for resistance to 4-nitroquinoline N-oxide, mediates transcriptional activation of the multidrug resistance transporter gene SNQ2. Mol Microbiol 29(5):1307-15
16) Le Crom S, et al.  (2002) New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system. Mol Cell Biol 22(8):2642-9
17) Miyahara K, et al.  (1996) yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae. Curr Genet 29(2):103-5
18) Lamping E, et al.  (2007) Characterization of three classes of membrane proteins involved in fungal azole resistance by functional hyperexpression in Saccharomyces cerevisiae. Eukaryot Cell 6(7):1150-65
19) Sipos G and Kuchler K  (2006) Fungal ATP-binding cassette (ABC) transporters in drug resistance & detoxification. Curr Drug Targets 7(4):471-81