SNP1/YIL061C Summary Help

Standard Name SNP1
Systematic Name YIL061C
Feature Type ORF, Verified
Description Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Gene Product Alias U1 70K 3 , U1-70K 1
Chromosomal Location
ChrIX:245559 to 244657 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SNP1 GO evidence and references
  View Computational GO annotations for SNP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Resources
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
155 total interaction(s) for 83 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 101
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 14
  • Biochemical Activity: 1
  • Co-purification: 1
  • Two-hybrid: 35

Genetic Interactions
  • Dosage Rescue: 1
  • Positive Genetic: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 300
Molecular Weight (Da) 34,447
Isoelectric Point (pI) 10.82
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIX:245559 to 244657 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..903 245559..244657 2011-02-03 1994-12-10
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001323
References cited on this page View Complete Literature Guide for SNP1
1) Hilleren PJ, et al.  (1995) The amino-terminal domain of yeast U1-70K is necessary and sufficient for function. Mol Cell Biol 15(11):6341-50
2) Neubauer G, et al.  (1997) Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry. Proc Natl Acad Sci U S A 94(2):385-90
3) Smith V and Barrell BG  (1991) Cloning of a yeast U1 snRNP 70K protein homologue: functional conservation of an RNA-binding domain between humans and yeast. EMBO J 10(9):2627-34
4) Kao HY and Siliciano PG  (1992) The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene. Nucleic Acids Res 20(15):4009-13
5) Gunderson SI, et al.  (1998) U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase. Mol Cell 1(2):255-64
6) Chen YC, et al.  (2010) Protein Arginine Methylation Facilitates Cotranscriptional Recruitment of Pre-mRNA Splicing Factors. Mol Cell Biol 30(21):5245-56
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76