SNF7/YLR025W Summary Help

Standard Name SNF7 1
Systematic Name YLR025W
Alias DID1 2 , VPS32 3 , 4 , RNS4 5 , VPL5 6
Feature Type ORF, Verified
Description One of four subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; ESCRT-III stands for endosomal sorting complex required for transport III (7, 8, 9 and see Summary Paragraph)
Name Description Sucrose NonFermenting 10
Chromosomal Location
ChrXII:194452 to 195174 | ORF Map | GBrowse
Gbrowse
Genetic position: 11.4 cM
Gene Ontology Annotations All SNF7 GO evidence and references
  View Computational GO annotations for SNF7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
reduction of function
unspecified
Large-scale survey
null
overexpression
Resources
162 total interaction(s) for 105 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 19
  • Co-localization: 3
  • FRET: 1
  • PCA: 9
  • Reconstituted Complex: 1
  • Two-hybrid: 75

Genetic Interactions
  • Dosage Growth Defect: 4
  • Dosage Rescue: 4
  • Negative Genetic: 1
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 7
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 5
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 240
Molecular Weight (Da) 26,987
Isoelectric Point (pI) 4.48
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:194452 to 195174 | ORF Map | GBrowse
SGD ORF map
Genetic position: 11.4 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..723 194452..195174 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004015
SUMMARY PARAGRAPH for SNF7

Most proteins targeted for degradation in the multivesicular body (MVB) pathway are monoubiquitinated, then passed through the series of highly-conserved multisubunit complexes known as ESCRT (Endosomal Sorting Complex Required for Transport). ESCRT I, II, and III are sequentially mobilized to endosomal membranes, where they direct protein sorting and MVB biogenesis, and play a crucial role in retrovirus budding (11).

The formation of ESCRT III - composed of subcomplexes Snf7p-Vps20p and Did4p-Vps24p - is transient and appears to be dependent on ESCRT II (12, 8). Snf7p-Vps20p effects the membrane association of ESCRT III, with Snf7p being required for localization of the Bro1p vacuolar protein sorting factor to endosomes (13, 14). Did4p-Vps24p is required for endosomal localization of the Doa4p ubiquitin hydrolase and the Vps4p AAA-type ATPase. Vps4p is necessary for the dissociation of ESCRT III (8, 2).

snf7 mutants are defective for vacuolar protein sorting, growth on medium containing raffinose plus antimycin A, derepressing transcription of SUC2, and sporulation (1, 15, 16). Null mutants in any of the four genes encoding ESCRT III proteins exhibit growth defects at high temperatures. Individual snf7 and vps20 null mutants also display hypersensitivity to calcium, lithium, and manganese ions, while did4 and vps24 null mutants are hypersensitive to manganese (17).

The four members of the ESCRT III complex are part of a family of small coiled-coil proteins which also includes includes Did2p, Moh1p, Vps60p, Candida albicans Snf7p, and human CHMP1, CHMP1.5, CHMP2 (BC-2, which is a putative breast adenocarcinoma marker), CHMP2.5 (CGI-84), CHMP3 (NEDF), CHMP4a, CHMP4b, CHMP5 (CGI-34), and CHMP6 (18, 2, 19, 20, 8, 21, 22, 23).

Last updated: 2006-03-01 Contact SGD

References cited on this page View Complete Literature Guide for SNF7
1) Vallier LG and Carlson M  (1991) New SNF genes, GAL11 and GRR1 affect SUC2 expression in Saccharomyces cerevisiae. Genetics 129(3):675-84
2) Amerik AY, et al.  (2000) The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell 11(10):3365-80
3) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
4) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
5) Unno K, et al.  (2005) Identification and characterization of rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae: Evidence for altered ambient response resulting in transportation of the secretory protein to vacuoles. FEMS Yeast Res 5(9):801-12
6) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
7) Babst M, et al.  (1998) The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function. EMBO J 17(11):2982-93
8) Babst M, et al.  (2002) Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell 3(2):271-82
9) Bowers K, et al.  (2004) Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic 5(3):194-210
10) Carlson M, et al.  (1981) Mutants of yeast defective in sucrose utilization. Genetics 98(1):25-40
11) Teo H, et al.  (2004) ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes. Dev Cell 7(4):559-69
12) Babst M, et al.  (2002) Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev Cell 3(2):283-9
13) Odorizzi G, et al.  (2003) Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae. J Cell Sci 116(Pt 10):1893-903
14) Boysen JH and Mitchell AP  (2006) Control of Bro1-domain protein Rim20 localization by external pH, ESCRT machinery, and the Saccharomyces cerevisiae Rim101 pathway. Mol Biol Cell 17(3):1344-53
15) Yeghiayan P, et al.  (1995) Molecular analysis of the SNF8 gene of Saccharomyces cerevisiae. Yeast 11(3):219-24
16) Tu J, et al.  (1993) Molecular and genetic analysis of the SNF7 gene in Saccharomyces cerevisiae. Genetics 135(1):17-23
17) Eguez L, et al.  (2004) Yeast Mn2+ transporter, Smf1p, is regulated by ubiquitin-dependent vacuolar protein sorting. Genetics 167(1):107-17
18) Ashrafi K, et al.  (1998) A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase. J Biol Chem 273(40):25864-74
19) Howard TL, et al.  (2001) CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci 114(Pt 13):2395-404
20) Kranz A, et al.  (2001) A family of small coiled-coil-forming proteins functioning at the late endosome in yeast. Mol Biol Cell 12(3):711-23
21) Whitley P, et al.  (2003) Identification of mammalian Vps24p as an effector of phosphatidylinositol 3,5-bisphosphate-dependent endosome compartmentalization. J Biol Chem 278(40):38786-95
22) Katoh K, et al.  (2003) The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting. J Biol Chem 278(40):39104-13
23) Cornet M, et al.  (2005) Deletions of endocytic components VPS28 and VPS32 affect growth at alkaline pH and virulence through both RIM101-dependent and RIM101-independent pathways in Candida albicans. Infect Immun 73(12):7977-87