SMT3/YDR510W Summary Help

Standard Name SMT3
Systematic Name YDR510W
Feature Type ORF, Verified
Description Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 (1, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Suppressor of Mif Two
Chromosomal Location
ChrIV:1469400 to 1469705 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SMT3 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 21 genes
Resources
Classical genetics
dominant negative
overexpression
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
1936 total interaction(s) for 1011 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1015
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 187
  • Biochemical Activity: 17
  • Co-crystal Structure: 4
  • Co-localization: 1
  • Co-purification: 2
  • PCA: 375
  • Protein-peptide: 3
  • Reconstituted Complex: 21
  • Two-hybrid: 101

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 5
  • Negative Genetic: 31
  • Phenotypic Suppression: 6
  • Positive Genetic: 6
  • Synthetic Growth Defect: 144
  • Synthetic Lethality: 7
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 101
Molecular Weight (Da) 11,597
Isoelectric Point (pI) 4.76
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1469400 to 1469705 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..306 1469400..1469705 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002918
References cited on this page View Complete Literature Guide for SMT3
1) Johnson ES and Blobel G  (1999) Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins. J Cell Biol 147(5):981-94
2) Takahashi Y, et al.  (1999) Smt3, a SUMO-1 homolog, is conjugated to Cdc3, a component of septin rings at the mother-bud neck in budding yeast. Biochem Biophys Res Commun 259(3):582-7
3) Biggins S, et al.  (2001) Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae. Genetics 159(2):453-70
4) Bachant J, et al.  (2002) The SUMO-1 isopeptidase Smt4 is linked to centromeric cohesion through SUMO-1 modification of DNA topoisomerase II. Mol Cell 9(6):1169-82
5) Hoege C, et al.  (2002) RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO. Nature 419(6903):135-41
6) Dieckhoff P, et al.  (2004) Smt3/SUMO and Ubc9 are required for efficient APC/C-mediated proteolysis in budding yeast. Mol Microbiol 51(5):1375-87
7) Stead K, et al.  (2003) Pds5p regulates the maintenance of sister chromatid cohesion and is sumoylated to promote the dissolution of cohesion. J Cell Biol 163(4):729-41
8) Matic I, et al.  (2008) Phosphorylation of SUMO-1 Occurs in Vivo and Is Conserved through Evolution. J Proteome Res 7(9):4050-4057
9) Rosonina E, et al.  (2010) SUMO functions in constitutive transcription and during activation of inducible genes in yeast. Genes Dev 24(12):1242-52