SMC4/YLR086W Summary

Standard Name	SMC4
Systematic Name	YLR086W
Feature Type	ORF, Verified
Description	Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate (1, 2, 3 and see Summary Paragraph)
Name Description	Structural Maintenance of Chromosomes

Chromosomal Location	ChrXII:302243 to 306499 \| ORF Map \| GBrowse

Gene Ontology Annotations All SMC4 GO evidence and references

	View Computational GO annotations for SMC4
Molecular Function
Manually curated	ATPase activity (ISS) chromatin binding (IDA, IGI)
Biological Process
Manually curated	mitotic chromosome condensation (IMP, TAS) mitotic sister chromatid segregation (TAS) tRNA gene clustering (IMP)
Cellular Component
Manually curated	nuclear condensin complex (IPI, TAS) colocalizes_with SMC loading complex (IDA)
High-throughput	nucleus (IDA)

Mutant phenotypes All SMC4 Phenotype evidence and references

Classical genetics
conditional	pre-tRNALeu localization: abnormal silencing: decreased
Large-scale survey
conditional	colony sectoring: increased
null	haploproficient inviable
reduction of function	competitive fitness: increased
repressible	cell cycle progression in S phase: abnormal chromosome/plasmid maintenance: decreased
Resources	PROPHECY \| PhenoM \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SMC4 Interaction evidence and references

	47 total interaction(s) for 29 unique genes/features.
Physical Interactions	Affinity Capture-MS: 22 Affinity Capture-RNA: 3 Affinity Capture-Western: 6 Biochemical Activity: 1 Co-purification: 1 Reconstituted Complex: 1 Two-hybrid: 8
Genetic Interactions	Negative Genetic: 1 Phenotypic Enhancement: 1 Positive Genetic: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SMC4 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:302243 to 306499 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4257	302243..306499	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004076

SUMMARY PARAGRAPH for SMC4

SMC4 is an essential gene that encodes a member of a ubiquitous family of chromosome-associated ATPases (4, 5, 6). SMC proteins are found in eukaryotes, prokaryotes, and archaea, and appear to play roles in chromosome dynamics (5, 6). In eukaryotes, SMC proteins form two kinds of heterodimers, corresponding to Smc1p-Smc3p and Smc2p-Smc4p in yeast (5, 6). The Smc2p-Smc4p heterodimer interacts with additional proteins, Brn1p, Ycg1p, and Loc7p, to form the yeast condensin complex(6, 5, 7, 8). Originally identified in Xenopus egg extracts, the condensin complex is required for chromosome condensation (9, 6, 5). Condensin complexes have been identified in many eukaryotes, including Drosophila, human, and S. pombe (5).

Last updated: 2000-05-04

References cited on this page View Complete Literature Guide for SMC4

1)	Stray JE and Lindsley JE (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278(28):26238-48
2)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
3)	Haeusler RA, et al. (2008) Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes. Genes Dev 22(16):2204-14
4)	Koshland D and Strunnikov A (1996) Mitotic chromosome condensation. Annu Rev Cell Dev Biol 12:305-33
5)	Hirano T (1999) SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates? Genes Dev 13(1):11-9
6)	Strunnikov AV and Jessberger R (1999) Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions. Eur J Biochem 263(1):6-13
7)	Ouspenski II, et al. (2000) Chromosome condensation factor Brn1p is required for chromatid separation in mitosis. Mol Biol Cell 11(4):1305-13
8)	Lavoie BD, et al. (2000) Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren. Mol Biol Cell 11(4):1293-304
9)	Hirano T, et al. (1997) Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein. Cell 89(4):511-21