SMC4/YLR086W Summary Help

Standard Name SMC4
Systematic Name YLR086W
Feature Type ORF, Verified
Description Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Structural Maintenance of Chromosomes
Chromosomal Location
ChrXII:302243 to 306499 | ORF Map | GBrowse
Gene Ontology Annotations All SMC4 GO evidence and references
  View Computational GO annotations for SMC4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
reduction of function
51 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 7
  • Biochemical Activity: 3
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 8

Genetic Interactions
  • Negative Genetic: 1
  • Phenotypic Enhancement: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 1,418
Molecular Weight (Da) 162,188
Isoelectric Point (pI) 6.13
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:302243 to 306499 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4257 302243..306499 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004076

SMC4 is an essential gene that encodes a member of a ubiquitous family of chromosome-associated ATPases (6, 7, 8). SMC proteins are found in eukaryotes, prokaryotes, and archaea, and appear to play roles in chromosome dynamics (7, 8). In eukaryotes, SMC proteins form two kinds of heterodimers, corresponding to Smc1p-Smc3p and Smc2p-Smc4p in yeast (7, 8). The Smc2p-Smc4p heterodimer interacts with additional proteins, Brn1p, Ycg1p, and Loc7p, to form the yeast condensin complex(8, 7, 9, 10). Originally identified in Xenopus egg extracts, the condensin complex is required for chromosome condensation (11, 8, 7). Condensin complexes have been identified in many eukaryotes, including Drosophila, human, and S. pombe (7).

Last updated: 2000-05-04 Contact SGD

References cited on this page View Complete Literature Guide for SMC4
1) Freeman L, et al.  (2000) The condensin complex governs chromosome condensation and mitotic transmission of rDNA. J Cell Biol 149(4):811-24
2) Stray JE and Lindsley JE  (2003) Biochemical analysis of the yeast condensin Smc2/4 complex: an ATPase that promotes knotting of circular DNA. J Biol Chem 278(28):26238-48
3) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
4) Yu HG and Koshland DE  (2003) Meiotic condensin is required for proper chromosome compaction, SC assembly, and resolution of recombination-dependent chromosome linkages. J Cell Biol 163(5):937-47
5) Haeusler RA, et al.  (2008) Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes. Genes Dev 22(16):2204-14
6) Koshland D and Strunnikov A  (1996) Mitotic chromosome condensation. Annu Rev Cell Dev Biol 12:305-33
7) Hirano T  (1999) SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates? Genes Dev 13(1):11-9
8) Strunnikov AV and Jessberger R  (1999) Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions. Eur J Biochem 263(1):6-13
9) Ouspenski II, et al.  (2000) Chromosome condensation factor Brn1p is required for chromatid separation in mitosis. Mol Biol Cell 11(4):1305-13
10) Lavoie BD, et al.  (2000) Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren. Mol Biol Cell 11(4):1293-304
11) Hirano T, et al.  (1997) Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein. Cell 89(4):511-21