SLX8/YER116C Summary Help

Standard Name SLX8 1
Systematic Name YER116C
Feature Type ORF, Verified
Description Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress (1, 2, 3, 4, 5)
Name Description Synthetic Lethal of unknown (X) function 1
Chromosomal Location
ChrV:396172 to 395348 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SLX8 GO evidence and references
  View Computational GO annotations for SLX8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
474 total interaction(s) for 315 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-Western: 4
  • Biochemical Activity: 8
  • Co-purification: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 226
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 1
  • Positive Genetic: 61
  • Synthetic Growth Defect: 92
  • Synthetic Lethality: 41
  • Synthetic Rescue: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 274
Molecular Weight (Da) 30,764
Isoelectric Point (pI) 4.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrV:396172 to 395348 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..825 396172..395348 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000918
References cited on this page View Complete Literature Guide for SLX8
1) Mullen JR, et al.  (2001) Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics 157(1):103-18
2) Wang Z, et al.  (2006) Genetic Analysis Connects SLX5 and SLX8 to the SUMO Pathway in Saccharomyces cerevisiae. Genetics 172(3):1499-509
3) Xie Y, et al.  (2007) The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation. J Biol Chem 282(47):34176-84
4) Ii T, et al.  (2007) The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity. Cell Cycle 6(22):2800-9
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76