SLM4/YBR077C Summary Help

Standard Name SLM4 1
Systematic Name YBR077C
Alias EGO3 2 , NIR1 3 , GSE1 4
Feature Type ORF, Verified
Description Component of the EGO and GSE complexes; essential for integrity and function of EGO; EGO is involved in the regulation of microautophagy and GSE is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 (1, 2, 3, 4, 5)
Name Description Synthetic Lethal with Mss4 1
Chromosomal Location
ChrII:392293 to 391805 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SLM4 GO evidence and references
  View Computational GO annotations for SLM4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 15 genes
Resources
Classical genetics
null
Large-scale survey
null
unspecified
Resources
105 total interaction(s) for 76 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 4
  • Co-crystal Structure: 2
  • Co-fractionation: 2
  • PCA: 6
  • Reconstituted Complex: 6
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 47
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 8
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 9
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 162
Molecular Weight (Da) 18,354
Isoelectric Point (pI) 4.77
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrII:392293 to 391805 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..489 392293..391805 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000281
References cited on this page View Complete Literature Guide for SLM4
1) Audhya A, et al.  (2004) Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton. EMBO J 23(19):3747-57
2) Dubouloz F, et al.  (2005) The TOR and EGO protein complexes orchestrate microautophagy in yeast. Mol Cell 19(1):15-26
3) Huang J, et al.  (2004) Finding new components of the target of rapamycin (TOR) signaling network through chemical genetics and proteome chips. Proc Natl Acad Sci U S A 101(47):16594-9
4) Gao M and Kaiser CA  (2006) A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast. Nat Cell Biol 8(7):657-67
5) Zhang T, et al.  (2012) Ego3 Functions as a Homodimer to Mediate the Interaction between Gtr1-Gtr2 and Ego1 in the EGO Complex to Activate TORC1. Structure 20(12):2151-60