Standard Name	SLM4 1
Systematic Name	YBR077C
Alias	EGO3 2 , NIR1 3 , GSE1 4
Feature Type	ORF, Verified
Description	Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 (1, 2, 3, 4)
Name Description	Synthetic Lethal with Mss4 1

Chromosomal Location	ChrII:392293 to 391805 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for SLM4
Molecular Function
Manually curated	phosphatidylinositol-3,4-bisphosphate binding (TAS)
Biological Process
Manually curated	microautophagy (IMP) signal transduction (IMP)
Cellular Component
Manually curated	EGO complex (IDA, IPI) fungal-type vacuole membrane (IDA) late endosome membrane (IDA)
High-throughput	cytoplasm (IDA) fungal-type vacuole membrane (IDA)

Classical genetics
null	metal resistance: decreased nutrient uptake: decreased replicative lifespan: abnormal resistance to sirolimus: decreased
Large-scale survey
null	auxotrophy glycogen accumulation: increased chitin deposition: increased competitive fitness: decreased fermentative growth: decreased rate heat sensitivity: increased resistance to wortmannin: decreased resistance to sirolimus: decreased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to benzo[a]pyrene: increased resistance to Calcofluor White: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to ethanol: decreased resistance to 5-fluorouracil: decreased resistance to 4-chlorophenol: decreased resistance to paromomycin: decreased resistance to caffeine: decreased resistance to hygromycin B: decreased resistance to doxorubicin: decreased resistance to benzo[a]pyrene: decreased resistance to sorbate: decreased sporulation efficiency: increased starvation resistance: decreased stress resistance: increased toxin resistance: decreased vacuolar morphology: abnormal viable
unspecified	resistance to l-azetidine-2-carboxylic acid: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	102 total interaction(s) for 74 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 2 Affinity Capture-Western: 4 Co-crystal Structure: 2 Co-fractionation: 2 PCA: 6 Reconstituted Complex: 6 Two-hybrid: 7
Genetic Interactions	Negative Genetic: 47 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Positive Genetic: 8 Synthetic Growth Defect: 5 Synthetic Lethality: 9 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrII:392293 to 391805 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..489 392293..391805 2011-02-03 1997-01-28
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain CLIB382 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000000281