SLD3/YGL113W Summary Help

Standard Name SLD3 1, 2
Systematic Name YGL113W
Feature Type ORF, Verified
Description Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress (2, 3, 4)
Name Description Synthetically Lethal with Dpb11-1 1
Chromosomal Location
ChrVII:295932 to 297938 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SLD3 GO evidence and references
  View Computational GO annotations for SLD3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
reduction of function
Large-scale survey
conditional
null
overexpression
Resources
96 total interaction(s) for 27 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Biochemical Activity: 4
  • Co-fractionation: 10
  • PCA: 1
  • Reconstituted Complex: 29
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Growth Defect: 9
  • Dosage Rescue: 5
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 668
Molecular Weight (Da) 77,297
Isoelectric Point (pI) 10.47
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:295932 to 297938 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2007 295932..297938 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003081
References cited on this page View Complete Literature Guide for SLD3
1) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9
2) Kamimura Y, et al.  (2001) Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae. EMBO J 20(8):2097-107
3) Takayama Y, et al.  (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76