SLD2/YKL108W Summary Help

Standard Name SLD2 1
Systematic Name YKL108W
Alias DRC1 2
Feature Type ORF, Verified
Description Single-stranded DNA origin-binding and annealing protein; required for initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; binds the Mcm2-7p complex to prevent inappropriate Mcm2-7p interaction with the GINS complex in G1; required for S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
Name Description Synthetically Lethal with Dpb11-1 1
Chromosomal Location
ChrXI:234426 to 235787 | ORF Map | GBrowse
Gene Ontology Annotations All SLD2 GO evidence and references
  View Computational GO annotations for SLD2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
93 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-Western: 12
  • Biochemical Activity: 5
  • Reconstituted Complex: 13
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 8
  • Dosage Lethality: 2
  • Dosage Rescue: 9
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 4

Expression Summary
Length (a.a.) 453
Molecular Weight (Da) 52,271
Isoelectric Point (pI) 9.79
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:234426 to 235787 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1362 234426..235787 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001591
References cited on this page View Complete Literature Guide for SLD2
1) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9
2) Wang H and Elledge SJ  (1999) DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 96(7):3824-9
3) Masumoto H, et al.  (2002) S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast. Nature 415(6872):651-5
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Tanaka S, et al.  (2007) CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast. Nature 445(7125):328-32
6) Zegerman P and Diffley JF  (2007) Phosphorylation of Sld2 and Sld3 by cyclin-dependent kinases promotes DNA replication in budding yeast. Nature 445(7125):281-5
7) Muramatsu S, et al.  (2010) CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol {varepsilon}, and GINS in budding yeast. Genes Dev 24(6):602-12
8) Kanter DM and Kaplan DL  (2011) Sld2 binds to origin single-stranded DNA and stimulates DNA annealing. Nucleic Acids Res 39(7):2580-92
9) Bruck I, et al.  (2011) Enabling association of the GINS protein tetramer with the mini chromosome maintenance (Mcm)2-7 protein complex by phosphorylated Sld2 protein and single-stranded origin DNA. J Biol Chem 286(42):36414-26
10) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
11) Bruck I and Kaplan DL  (2014) The replication initiation protein Sld2 regulates helicase assembly. J Biol Chem 289(4):1948-59