SLA2/YNL243W Summary

SLA2/YNL243W Summary

Standard Name	SLA2 1
Systematic Name	YNL243W
Alias	END4 2 , 3 , MOP2 4
Feature Type	ORF, Verified
Description	Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo (5, 6, 7, 8, 9)
Name Description	Synthetic Lethal with ABP1 1

Chromosomal Location	ChrXIV:188051 to 190957 \| ORF Map \| GBrowse

Gene Ontology Annotations All SLA2 GO evidence and references

	View Computational GO annotations for SLA2
Molecular Function
Manually curated	protein binding, bridging (IGI, IPI)
Biological Process
Manually curated	actin filament organization (IMP) bipolar cellular bud site selection (TAS) endocytosis (IMP) exocytosis (TAS) fungal-type cell wall organization (TAS)
Cellular Component
Manually curated	actin cortical patch (IDA) incipient cellular bud site (IDA)
High-throughput	mating projection tip (IDA)

Mutant phenotypes All SLA2 Phenotype evidence and references

Classical genetics
conditional	cell wall morphology: abnormal endocytosis: absent endocytosis: decreased endomembrane system morphology: abnormal heat sensitivity: increased killer toxin resistance: increased Pma1p accumulation: decreased septum formation: abnormal
null	actin cytoskeleton morphology: abnormal bipolar budding pattern: decreased cell shape: abnormal cell size: increased heat sensitivity: increased inviable invertase secretion: decreased Bgl2p accumulation: increased Snc1p accumulation: increased Clc1p distribution: abnormal Sla1p distribution: abnormal pseudohyphal growth: absent resistance to latrunculin A: decreased vegetative growth: decreased rate viable
Large-scale survey
null	acid pH resistance: decreased cell size: increased fermentative growth: decreased rate haploproficient heat sensitivity: increased lipid particle morphology: abnormal resistance to sulfanilamide: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to ethanol: decreased resistance to methanol: decreased resistance to propan-1-ol: decreased resistance to (S)-lactic acid: decreased resistance to hydrogen chloride: decreased resistance to boric acid: decreased resistance to chloroquine: decreased telomere length: decreased transposable element transposition: decreased vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SLA2 Interaction evidence and references

	153 total interaction(s) for 106 unique genes/features.
Physical Interactions	Affinity Capture-MS: 17 Affinity Capture-RNA: 1 Affinity Capture-Western: 6 Biochemical Activity: 1 Co-fractionation: 3 Co-localization: 1 PCA: 12 Protein-peptide: 1 Reconstituted Complex: 3 Two-hybrid: 32
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 1 Phenotypic Enhancement: 12 Phenotypic Suppression: 6 Synthetic Growth Defect: 11 Synthetic Lethality: 44
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SLA2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXIV:188051 to 190957 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 2011-02-03

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2907	188051..190957	2011-02-03	2011-02-03

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000005187

References cited on this page View Complete Literature Guide for SLA2

1)	Holtzman DA, et al. (1993) Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol 122(3):635-44
2)	Wesp A, et al. (1997) End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae. Mol Biol Cell 8(11):2291-306
3)	Raths S, et al. (1993) end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J Cell Biol 120(1):55-65
4)	Na S, et al. (1995) MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae. J Biol Chem 270(12):6815-23
5)	Pruyne D and Bretscher A (2000) Polarization of cell growth in yeast. J Cell Sci 113 ( Pt 4):571-85
6)	Yang S, et al. (1999) Sla2p is associated with the yeast cortical actin cytoskeleton via redundant localization signals. Mol Biol Cell 10(7):2265-83
7)	Munn AL (2001) Molecular requirements for the internalisation step of endocytosis: insights from yeast. Biochim Biophys Acta 1535(3):236-57
8)	Palecek J, et al. (2001) Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Biochem Biophys Res Commun 282(5):1244-50
9)	Baggett JJ, et al. (2003) The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae. Genetics 165(4):1661-74