SKN7/YHR206W Summary Help

Standard Name SKN7 1
Systematic Name YHR206W
Alias BRY1 2 , POS9 3
Feature Type ORF, Verified
Description Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication (4, 5, 6, 7, 8, 9)
Name Description Suppressor of Kre Null 10
Chromosomal Location
ChrVIII:512732 to 514600 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SKN7 GO evidence and references
  View Computational GO annotations for SKN7
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 167 genes
Regulators 2 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
297 total interaction(s) for 198 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 8
  • Biochemical Activity: 5
  • PCA: 2
  • Protein-peptide: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 30

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 11
  • Negative Genetic: 172
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 3
  • Positive Genetic: 13
  • Synthetic Growth Defect: 24
  • Synthetic Lethality: 3
  • Synthetic Rescue: 7

Resources
Expression Summary
histogram
Resources
Length (a.a.) 622
Molecular Weight (Da) 69,202
Isoelectric Point (pI) 7.04
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVIII:512732 to 514600 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1869 512732..514600 2005-11-07 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001249
References cited on this page View Complete Literature Guide for SKN7
1) Brown JL, et al.  (1993) SKN7, a yeast multicopy suppressor of a mutation affecting cell wall beta-glucan assembly, encodes a product with domains homologous to prokaryotic two-component regulators and to heat shock transcription factors. J Bacteriol 175(21):6908-15
2) Morgan BA, et al.  (1995) A yeast transcription factor bypassing the requirement for SBF and DSC1/MBF in budding yeast has homology to bacterial signal transduction proteins. EMBO J 14(22):5679-89
3) Krems B, et al.  (1996) The response regulator-like protein Pos9/Skn7 of Saccharomyces cerevisiae is involved in oxidative stress resistance. Curr Genet 29(4):327-34
4) Raitt DC, et al.  (2000) The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress. Mol Biol Cell 11(7):2335-47
5) Ikner A and Shiozaki K  (2005) Yeast signaling pathways in the oxidative stress response. Mutat Res 569(1-2):13-27
6) Janiak-Spens F, et al.  (2005) Kinetic analysis of YPD1-dependent phosphotransfer reactions in the yeast osmoregulatory phosphorelay system. Biochemistry 44(1):377-86
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Hanlon SE, et al.  (2011) The Stress Response Factors Yap6, Cin5, Phd1, and Skn7 Direct Targeting of the Conserved Co-Repressor Tup1-Ssn6 in S. cerevisiae. PLoS One 6(4):e19060
9) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
10) Roemer T, et al.  (1993) SKN1 and KRE6 define a pair of functional homologs encoding putative membrane proteins involved in beta-glucan synthesis. Mol Cell Biol 13(7):4039-48
11) Li S, et al.  (2002) The eukaryotic two-component histidine kinase Sln1p regulates OCH1 via the transcription factor, Skn7p. Mol Biol Cell 13(2):412-24
12) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
13) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
14) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104