SKM1/YOL113W Summary Help

Standard Name SKM1 1
Systematic Name YOL113W
Feature Type ORF, Verified
Description Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication (1, 2, 3, 4)
Name Description STE20/PAK homologous Kinase related to Morphogenesis 1
Chromosomal Location
ChrXV:104326 to 106293 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SKM1 GO evidence and references
  View Computational GO annotations for SKM1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
Regulators 104 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
115 total interaction(s) for 106 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 39
  • PCA: 1
  • Protein-peptide: 2
  • Reconstituted Complex: 8
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Rescue: 1
  • Negative Genetic: 33
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 655
Molecular Weight (Da) 75,331
Isoelectric Point (pI) 9.25
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:104326 to 106293 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1968 104326..106293 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005473
References cited on this page View Complete Literature Guide for SKM1
1) Martin H, et al.  (1997) Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK-like protein kinase. Mol Microbiol 23(3):431-44
2) Richman TJ, et al.  (1999) The Cdc42p GTPase is involved in a G2/M morphogenetic checkpoint regulating the apical-isotropic switch and nuclear division in yeast. J Biol Chem 274(24):16861-70
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Lin M, et al.  (2009) The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway. Mol Biol Cell 20(22):4826-37