SIZ1/YDR409W Summary Help

Standard Name SIZ1 1
Systematic Name YDR409W
Alias ULL1 2
Feature Type ORF, Verified
Description SUMO/Smt3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring; SIZ1 has a paralog, NFI1, that arose from the whole genome duplication (3, 4, 5, 6)
Name Description SAP and mIZ-finger domain 1
Chromosomal Location
ChrIV:1289406 to 1292120 | ORF Map | GBrowse
Gene Ontology Annotations All SIZ1 GO evidence and references
  View Computational GO annotations for SIZ1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
Large-scale survey
241 total interaction(s) for 140 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 84
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 12
  • Biochemical Activity: 13
  • Co-crystal Structure: 1
  • Reconstituted Complex: 7
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 9
  • Phenotypic Enhancement: 18
  • Phenotypic Suppression: 20
  • Positive Genetic: 3
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 7
  • Synthetic Rescue: 21

Expression Summary
Length (a.a.) 904
Molecular Weight (Da) 100,795
Isoelectric Point (pI) 6.91
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1289406 to 1292120 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2715 1289406..1292120 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002817
References cited on this page View Complete Literature Guide for SIZ1
1) Strunnikov AV, et al.  (2001) Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation. Genetics 158(1):95-107
2) Takahashi Y, et al.  (2001) Yeast Ull1/Siz1 is a novel SUMO1/Smt3 ligase for septin components and functions as an adaptor between conjugating enzyme and substrates. J Biol Chem 276(52):48973-7
3) Takahashi Y, et al.  (2001) A novel factor required for the SUMO1/Smt3 conjugation of yeast septins. Gene 275(2):223-31
4) Hochstrasser M  (2001) SP-RING for SUMO: new functions bloom for a ubiquitin-like protein. Cell 107(1):5-8
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Novoselova TV, et al.  (2013) SUMOylation regulates the homologous to E6-AP carboxyl terminus (HECT) ubiquitin ligase Rsp5p. J Biol Chem 288(15):10308-17